7znx: Difference between revisions
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The | ==Crystal structure of cocaprin 1, inhibitor of cysteine and aspartic proteases from Coprinopsis cinerea== | ||
<StructureSection load='7znx' size='340' side='right'caption='[[7znx]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7znx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZNX FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7znx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7znx OCA], [https://pdbe.org/7znx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7znx RCSB], [https://www.ebi.ac.uk/pdbsum/7znx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7znx ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A8PCJ3_COPC7 A8PCJ3_COPC7] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We introduce a new family of fungal protease inhibitors with beta-trefoil fold from the mushroom Coprinopsis cinerea, named cocaprins, which inhibit both cysteine and aspartic proteases. Two cocaprin-encoding genes are differentially expressed in fungal tissues. One is highly transcribed in vegetative mycelium and the other in the stipes of mature fruiting bodies. Cocaprins are small proteins (15 kDa) with acidic isoelectric points that form dimers. The three-dimensional structure of cocaprin 1 showed similarity to fungal beta-trefoil lectins. Cocaprins inhibit plant C1 family cysteine proteases with K(i) in the micromolar range, but do not inhibit the C13 family protease legumain, which distinguishes them from mycocypins. Cocaprins also inhibit the aspartic protease pepsin with K(i) in the low micromolar range. Mutagenesis revealed that the beta2-beta3 loop is involved in the inhibition of cysteine proteases and that the inhibitory reactive sites for aspartic and cysteine proteases are located at different positions on the protein. Their biological function is thought to be the regulation of endogenous proteolytic activities or in defense against fungal antagonists. Cocaprins are the first characterized aspartic protease inhibitors with beta-trefoil fold from fungi, and demonstrate the incredible plasticity of loop functionalization in fungal proteins with beta-trefoil fold. | |||
Cocaprins, beta-Trefoil Fold Inhibitors of Cysteine and Aspartic Proteases from Coprinopsis cinerea.,Renko M, Zupan T, Plaza DF, Schmieder SS, Perisic Nanut M, Kos J, Turk D, Kunzler M, Sabotic J Int J Mol Sci. 2022 Apr 28;23(9):4916. doi: 10.3390/ijms23094916. PMID:35563308<ref>PMID:35563308</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Renko | <div class="pdbe-citations 7znx" style="background-color:#fffaf0;"></div> | ||
[[Category: Sabotic | == References == | ||
[[Category: Turk | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Coprinopsis cinerea]] | |||
[[Category: Large Structures]] | |||
[[Category: Renko M]] | |||
[[Category: Sabotic J]] | |||
[[Category: Turk D]] | |||
Latest revision as of 16:33, 1 February 2024
Crystal structure of cocaprin 1, inhibitor of cysteine and aspartic proteases from Coprinopsis cinereaCrystal structure of cocaprin 1, inhibitor of cysteine and aspartic proteases from Coprinopsis cinerea
Structural highlights
FunctionPublication Abstract from PubMedWe introduce a new family of fungal protease inhibitors with beta-trefoil fold from the mushroom Coprinopsis cinerea, named cocaprins, which inhibit both cysteine and aspartic proteases. Two cocaprin-encoding genes are differentially expressed in fungal tissues. One is highly transcribed in vegetative mycelium and the other in the stipes of mature fruiting bodies. Cocaprins are small proteins (15 kDa) with acidic isoelectric points that form dimers. The three-dimensional structure of cocaprin 1 showed similarity to fungal beta-trefoil lectins. Cocaprins inhibit plant C1 family cysteine proteases with K(i) in the micromolar range, but do not inhibit the C13 family protease legumain, which distinguishes them from mycocypins. Cocaprins also inhibit the aspartic protease pepsin with K(i) in the low micromolar range. Mutagenesis revealed that the beta2-beta3 loop is involved in the inhibition of cysteine proteases and that the inhibitory reactive sites for aspartic and cysteine proteases are located at different positions on the protein. Their biological function is thought to be the regulation of endogenous proteolytic activities or in defense against fungal antagonists. Cocaprins are the first characterized aspartic protease inhibitors with beta-trefoil fold from fungi, and demonstrate the incredible plasticity of loop functionalization in fungal proteins with beta-trefoil fold. Cocaprins, beta-Trefoil Fold Inhibitors of Cysteine and Aspartic Proteases from Coprinopsis cinerea.,Renko M, Zupan T, Plaza DF, Schmieder SS, Perisic Nanut M, Kos J, Turk D, Kunzler M, Sabotic J Int J Mol Sci. 2022 Apr 28;23(9):4916. doi: 10.3390/ijms23094916. PMID:35563308[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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