7p9d: Difference between revisions
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<StructureSection load='7p9d' size='340' side='right'caption='[[7p9d]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='7p9d' size='340' side='right'caption='[[7p9d]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7p9d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P9D FirstGlance]. <br> | <table><tr><td colspan='2'>[[7p9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P9D FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9d OCA], [https://pdbe.org/7p9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9d RCSB], [https://www.ebi.ac.uk/pdbsum/7p9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9d ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9d OCA], [https://pdbe.org/7p9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9d RCSB], [https://www.ebi.ac.uk/pdbsum/7p9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A2K3E888_CHLRE A0A2K3E888_CHLRE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 7p9d" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7p9d" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Thioredoxin reductase 3D structures|Thioredoxin reductase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Chlamydomonas reinhardtii]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hippler M]] | |||
[[Category: Hippler | [[Category: Kuemmel D]] | ||
[[Category: Kuemmel | [[Category: Marchetti GM]] | ||
[[Category: Marchetti | [[Category: Singh RK]] | ||
[[Category: Singh | |||
Revision as of 16:02, 1 February 2024
Crystal structure of Chlamydomonas reinhardtii NADPH Dependent Thioredoxin Reductase 1 domainCrystal structure of Chlamydomonas reinhardtii NADPH Dependent Thioredoxin Reductase 1 domain
Structural highlights
FunctionPublication Abstract from PubMedIn plant chloroplasts, thiol regulation is driven by two systems. One relies on the activity of thioredoxins through their light dependent reduction by ferredoxin via a ferredoxin-thioredoxin reductase (FTR). In the other system, a NADPH-dependent redox regulation is driven by a NADPH-thioredoxin reductase C (NTRC). While the thioredoxin system has been deeply studied, a more thorough understanding of the function of this plant specific NTRC is desirable. NTRC is a single polypeptide harbouring a thioredoxin domain (Trx) at the C-terminus of a NADPH-dependent Thioredoxin reductase (TrxR). To provide functional and structural insights, we studied the crystal structure of the TrxR domain of the NTRC from Chlamydomonas reinhardtii (CrNTRC, Cre01.g054150.t1.2) and its Cys136Ser (C136S) mutant, which is characterized by the mutation of the resolving cysteine in the active site of the TrxR domain. Furthermore, we confirmed the role of NTRC as electron donor for 2-Cys peroxiredoxin (PRX) also in C. reinhardtii. The structural data of TrxR were employed to develop a scheme of action which addresses electron transfer between TrxR and Trx of NTRC and between NTRC and its substrates. Structural analysis revealed a novel conformation of the NTRC reductase domain from Chlamydomonas reinhardtii.,Marchetti GM, Fusser F, Singh RK, Brummel M, Koch O, Kummel D, Hippler M J Struct Biol. 2021 Dec 30;214(1):107829. doi: 10.1016/j.jsb.2021.107829. PMID:34974142[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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