7aj0: Difference between revisions
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<StructureSection load='7aj0' size='340' side='right'caption='[[7aj0]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='7aj0' size='340' side='right'caption='[[7aj0]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7aj0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_sp. | <table><tr><td colspan='2'>[[7aj0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_sp._MB47 Pseudoalteromonas sp. MB47]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AJ0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aj0 OCA], [https://pdbe.org/7aj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aj0 RCSB], [https://www.ebi.ac.uk/pdbsum/7aj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aj0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aj0 OCA], [https://pdbe.org/7aj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aj0 RCSB], [https://www.ebi.ac.uk/pdbsum/7aj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aj0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</div> | </div> | ||
<div class="pdbe-citations 7aj0" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7aj0" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Sulfatase 3D structures|Sulfatase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Pseudoalteromonas sp. | [[Category: Pseudoalteromonas sp. MB47]] | ||
[[Category: Czjzek | [[Category: Czjzek M]] | ||
[[Category: Meyer | [[Category: Meyer AS]] | ||
[[Category: Mikkelsen | [[Category: Mikkelsen MD]] | ||
[[Category: Roret | [[Category: Roret T]] | ||
Latest revision as of 15:10, 1 February 2024
Crystal structure of PsFucS1 sulfatase from Pseudoalteromonas sp.Crystal structure of PsFucS1 sulfatase from Pseudoalteromonas sp.
Structural highlights
Publication Abstract from PubMedFucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization and the first crystal structure of a prokaryotic sulfatase, PsFucS1, belonging to sulfatase subfamily S1_13, able to release sulfate from fucoidan oligosaccharides. PsFucS1 was identified in the genome of a Pseudoalteromonas sp. isolated from sea cucumber gut. PsFucS1 (57 kDa) is Ca(2+) dependent and has an unusually high optimal temperature (68 degrees C) and thermostability. Further, the PsFucS1 displays a unique quaternary hexameric structure comprising a tight trimeric dimer complex. The structural data imply that this hexamer formation results from an uncommon interaction of each PsFucS1 monomer that is oriented perpendicular to the common dimer interface (~ 1500 A(2)) that can be found in analogous sulfatases. The uncommon interaction involves interfacing (1246 A(2)) through a bundle of alpha-helices in the N-terminal domain to form a trimeric ring structure. The high thermostability may be related to this unusual quaternary hexameric structure formation that is suggested to represent a novel protein thermostabilization mechanism. A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure.,Mikkelsen MD, Cao HTT, Roret T, Rhein-Knudsen N, Holck J, Tran VTT, Nguyen TT, Tran VHN, Lezyk MJ, Muschiol J, Pham TD, Czjzek M, Meyer AS Sci Rep. 2021 Sep 30;11(1):19523. doi: 10.1038/s41598-021-98588-3. PMID:34593864[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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