6zn9: Difference between revisions
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<StructureSection load='6zn9' size='340' side='right'caption='[[6zn9]], [[Resolution|resolution]] 2.72Å' scene=''> | <StructureSection load='6zn9' size='340' side='right'caption='[[6zn9]], [[Resolution|resolution]] 2.72Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6zn9]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[6zn9]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bdellovibrio_bacteriovorus_HD100 Bdellovibrio bacteriovorus HD100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN9 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn9 OCA], [https://pdbe.org/6zn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn9 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn9 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn9 OCA], [https://pdbe.org/6zn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn9 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6MM15_BDEBA Q6MM15_BDEBA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6zn9" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6zn9" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Bdellovibrio bacteriovorus HD100]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Harding | [[Category: Harding CJ]] | ||
[[Category: Lovering | [[Category: Lovering AL]] | ||
Latest revision as of 14:54, 1 February 2024
MaeB PTA domain apoproteinMaeB PTA domain apoprotein
Structural highlights
FunctionPublication Abstract from PubMedBacterial hybrid malic enzymes (MaeB grouping, multidomain) catalyse the transformation of malate to pyruvate, and are a major contributor to cellular reducing power and carbon flux. Distinct from other malic enzyme subtypes, the hybrid enzymes are regulated by acetyl-CoA, a molecular indicator of the metabolic state of the cell. Here we solve the structure of a MaeB protein, which reveals hybrid enzymes use the appended phosphotransacetylase (PTA) domain to form a hexameric sensor that communicates acetyl-CoA occupancy to the malic enzyme active site, 60 A away. We demonstrate that allostery is governed by a large-scale rearrangement that rotates the catalytic subunits 70 degrees between the two states, identifying MaeB as a new model enzyme for the study of ligand-induced conformational change. Our work provides the mechanistic basis for metabolic control of hybrid malic enzymes, and identifies inhibition-insensitive variants that may find utility in synthetic biology. A rotary mechanism for allostery in bacterial hybrid malic enzymes.,Harding CJ, Cadby IT, Moynihan PJ, Lovering AL Nat Commun. 2021 Feb 23;12(1):1228. doi: 10.1038/s41467-021-21528-2. PMID:33623032[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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