1pue: Difference between revisions

Revision as of 05:29, 3 May 2008

PU.1 ETS DOMAIN-DNA COMPLEX

OverviewOverview

The Ets family of transcription factors, of which there are now about 35 members regulate gene expression during growth and development. They share a conserved domain of around 85 amino acids which binds as a monomer to the DNA sequence 5'-C/AGGAA/T-3'. We have determined the crystal structure of an ETS domain complexed with DNA, at 2.3-A resolution. The domain is similar to alpha + beta (winged) 'helix-turn-helix' proteins and interacts with a ten-base-pair region of duplex DNA which takes up a uniform curve of 8 degrees. The domain contacts the DNA by a novel loop-helix-loop architecture. Four of amino acids that directly interact with the DNA are highly conserved: two arginines from the recognition helix lying in the major groove, one lysine from the 'wing' that binds upstream of the core GGAA sequence, and another lysine, from the 'turn' of the 'helix-turn-helix' motif, which binds downstream and on the opposite strand.

About this StructureAbout this Structure

1PUE is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex., Kodandapani R, Pio F, Ni CZ, Piccialli G, Klemsz M, McKercher S, Maki RA, Ely KR, Nature. 1996 Apr 4;380(6573):456-60. PMID:8602247 Page seeded by OCA on Sat May 3 05:29:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1pue.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1pue", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pue OCA], [http://www.ebi.ac.uk/pdbsum/1pue PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pue RCSB]</span>
-}}
 '''PU.1 ETS DOMAIN-DNA COMPLEX'''
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 [[Category: Piccialli, G.]]
 [[Category: Pio, F.]]
-[[Category: activator]]
+[[Category: Activator]]
-[[Category: complex (transcription regulating/dna)]]
+[[Category: Dna- binding]]
-[[Category: dna- binding]]
+[[Category: Nuclear protein]]
-[[Category: nuclear protein]]
+[[Category: Oncogene]]
-[[Category: oncogene]]
+[[Category: Transforming protein]]
-[[Category: transforming protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:29:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:41 2008''