3j08: Difference between revisions

Revision as of 17:12, 24 January 2024

High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopAHigh resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA

3j08, resolution 10.00Å

Structural highlights

3j08 is a 2 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 10Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

COPA_ARCFU Probably involved in copper and silver export.^[1]

Publication Abstract from PubMed

CopA uses ATP to pump Cu(+) across cell membranes. X-ray crystallography has defined atomic structures of several related P-type ATPases. We have determined a structure of CopA at 10 A resolution by cryo-electron microscopy of a new crystal form and used computational molecular docking to study the interactions between the N-terminal metal-binding domain (NMBD) and other elements of the molecule. We found that the shorter-chain lipids used to produce these crystals are associated with movements of the cytoplasmic domains, with a novel dimer interface and with disordering of the NMBD, thus offering evidence for the transience of its interaction with the other cytoplasmic domains. Docking identified a binding site that matched the location of the NMBD in our previous structure by cryo-electron microscopy, allowing a more detailed view of its binding configuration and further support for its role in autoinhibition.

The Architecture of CopA from Archeaoglobus fulgidus Studied by Cryo-Electron Microscopy and Computational Docking.,Allen GS, Wu CC, Cardozo T, Stokes DL Structure. 2011 Aug 3. PMID:21820315^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mandal AK, Cheung WD, Arguello JM. Characterization of a thermophilic P-type Ag+/Cu+-ATPase from the extremophile Archaeoglobus fulgidus. J Biol Chem. 2002 Mar 1;277(9):7201-8. Epub 2001 Dec 26. PMID:11756450 doi:10.1074/jbc.M109964200
↑ Allen GS, Wu CC, Cardozo T, Stokes DL. The Architecture of CopA from Archeaoglobus fulgidus Studied by Cryo-Electron Microscopy and Computational Docking. Structure. 2011 Aug 3. PMID:21820315 doi:10.1016/j.str.2011.05.014

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OCA

[1] Mandal AK, Cheung WD, Arguello JM. Characterization of a thermophilic P-type Ag+/Cu+-ATPase from the extremophile Archaeoglobus fulgidus. J Biol Chem. 2002 Mar 1;277(9):7201-8. Epub 2001 Dec 26. PMID:11756450 doi:10.1074/jbc.M109964200

[2] Allen GS, Wu CC, Cardozo T, Stokes DL. The Architecture of CopA from Archeaoglobus fulgidus Studied by Cryo-Electron Microscopy and Computational Docking. Structure. 2011 Aug 3. PMID:21820315 doi:10.1016/j.str.2011.05.014

[1]

[2]

@@ Line 3: / Line 3: @@
 <SX load='3j08' size='340' side='right' viewer='molstar' caption='[[3j08]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J08 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J08 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">copA, pacS, AF_0473 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 ARCFL])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j08 OCA], [https://pdbe.org/3j08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j08 RCSB], [https://www.ebi.ac.uk/pdbsum/3j08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j08 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/COPA_ARCFU COPA_ARCFU]] Probably involved in copper and silver export.<ref>PMID:11756450</ref>
+[https://www.uniprot.org/uniprot/COPA_ARCFU COPA_ARCFU] Probably involved in copper and silver export.<ref>PMID:11756450</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </SX>
-[[Category: Arcfl]]
+[[Category: Archaeoglobus fulgidus]]
 [[Category: Large Structures]]
-[[Category: Allen, G S]]
+[[Category: Allen GS]]
-[[Category: Cardozo, T]]
+[[Category: Cardozo T]]
-[[Category: Stokes, D L]]
+[[Category: Stokes DL]]
-[[Category: Wu, C]]
+[[Category: Wu C]]
-[[Category: Adenosine triphosphatase]]
-[[Category: Archaeal protein]]
-[[Category: Cation transport protein]]
-[[Category: Copa]]
-[[Category: Copper transporter]]
-[[Category: Cryoelectron microscopy]]
-[[Category: Hydrolase]]
-[[Category: Metal transport]]
-[[Category: P-type atpase]]

3j08: Difference between revisions

Revision as of 17:12, 24 January 2024

High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopAHigh resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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3j08: Difference between revisions

Revision as of 17:12, 24 January 2024

High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopAHigh resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Search