1prq: Difference between revisions

Revision as of 05:24, 3 May 2008

ACANTHAMOEBA CASTELLANII PROFILIN IA

OverviewOverview

Profilin-I from Acanthamoeba castellanii is a 13-kDa protein that binds actin and poly-l-proline. The native protein has been crystallized in two different but closely related forms. The second form proved more amenable to three-dimensional structural determination using heavy-atom isomorphous methods to obtain crystallographic phase information. We used the second crystal structure as a test molecule in the molecular replacement procedure to determine the structure of the first crystal form of profilin-I. More residues participate in crystal lattice contacts in the first crystal form than in the second. The two crystal forms differ significantly in the C-terminal helix that interacts with actin and in the loop preceding this helix. Coordinates of some main chain atoms here differ by about 1.0 A, and side chain atoms differ by more than 2.0 A.

About this StructureAbout this Structure

1PRQ is a Single protein structure of sequence from Acanthamoeba castellanii. Full crystallographic information is available from OCA.

ReferenceReference

Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii., Liu S, Fedorov AA, Pollard TD, Lattman EE, Almo SC, Magnus KA, J Struct Biol. 1998 Sep;123(1):22-9. PMID:9774541 Page seeded by OCA on Sat May 3 05:24:36 2008

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OCA

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 '''ACANTHAMOEBA CASTELLANII PROFILIN IA'''
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 [[Category: Pollard, T D.]]
 [[Category: Way, M.]]
-[[Category: actin-binding protein]]
+[[Category: Actin-binding protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:24:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:03:39 2008''