6teo: Difference between revisions
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<StructureSection load='6teo' size='340' side='right'caption='[[6teo]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='6teo' size='340' side='right'caption='[[6teo]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6teo]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6teo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TEO FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6teo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6teo OCA], [https://pdbe.org/6teo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6teo RCSB], [https://www.ebi.ac.uk/pdbsum/6teo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6teo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/SN114_YEAST SN114_YEAST] Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6teo" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6teo" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Absmeier | [[Category: Saccharomyces cerevisiae S288C]] | ||
[[Category: Ganichkin | [[Category: Absmeier E]] | ||
[[Category: Jia | [[Category: Ganichkin O]] | ||
[[Category: Loll | [[Category: Jia J]] | ||
[[Category: Wahl | [[Category: Loll B]] | ||
[[Category: Wahl MC]] | |||
Revision as of 16:00, 24 January 2024
Crystal structure of a yeast Snu114-Prp8 complexCrystal structure of a yeast Snu114-Prp8 complex
Structural highlights
FunctionSN114_YEAST Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP. Publication Abstract from PubMedThe single G protein of the spliceosome, Snu114, has been proposed to facilitate splicing as a molecular motor or as a regulatory G protein. However, available structures of spliceosomal complexes show Snu114 in the same GTP-bound state, and presently no Snu114 GTPase-regulatory protein is known. We determined a crystal structure of Snu114 with a Snu114-binding region of the Prp8 protein, in which Snu114 again adopts the same GTP-bound conformation seen in spliceosomes. Snu114 and the Snu114-Prp8 complex co-purified with endogenous GTP. Snu114 exhibited weak, intrinsic GTPase activity that was abolished by the Prp8 Snu114-binding region. Exchange of GTP-contacting residues in Snu114, or of Prp8 residues lining the Snu114 GTP-binding pocket, led to temperature-sensitive yeast growth and affected the same set of splicing events in vivo. Consistent with dynamic Snu114-mediated protein interactions during splicing, our results suggest that the Snu114-GTP-Prp8 module serves as a relay station during spliceosome activation and disassembly, but that GTPase activity may be dispensable for splicing. A Snu114-GTP-Prp8 module forms a relay station for efficient splicing in yeast.,Jia J, Ganichkin OM, Preussner M, Absmeier E, Alings C, Loll B, Heyd F, Wahl MC Nucleic Acids Res. 2020 Mar 20. pii: 5810484. doi: 10.1093/nar/gkaa182. PMID:32196113[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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