6sbc: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='6sbc' size='340' side='right'caption='[[6sbc]], [[Resolution|resolution]] 1.35Å' scene=''> | <StructureSection load='6sbc' size='340' side='right'caption='[[6sbc]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6sbc]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6sbc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_sp._PCC_10023 Scytonema sp. PCC 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SBC FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=L4H:farnesyl+dihydroxybenzoate'>L4H</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbc OCA], [https://pdbe.org/6sbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sbc RCSB], [https://www.ebi.ac.uk/pdbsum/6sbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbc ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A2D1CM82_9CYAN A0A2D1CM82_9CYAN] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 23: | Line 24: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Scytonema sp. | [[Category: Scytonema sp. PCC 10023]] | ||
[[Category: Cahn | [[Category: Cahn JKB]] | ||
[[Category: Ecker | [[Category: Ecker F]] | ||
[[Category: Groll | [[Category: Groll M]] | ||
[[Category: Leopold-Messer | [[Category: Leopold-Messer S]] | ||
[[Category: Moosmann | [[Category: Moosmann P]] | ||
[[Category: Piel | [[Category: Piel J]] | ||
Latest revision as of 15:38, 24 January 2024
Structure of type II terpene cyclase MstE from Scytonema in complex with farnesyl dihydroxybenzoateStructure of type II terpene cyclase MstE from Scytonema in complex with farnesyl dihydroxybenzoate
Structural highlights
FunctionPublication Abstract from PubMedClass II terpene cyclases, such as oxidosqualene and squalene-hopene cyclases, catalyse some of the most complex polycyclization reactions. They minimally exhibit a beta,gamma-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the corresponding class II counterparts have not been previously reported. Here we present high-resolution X-ray structures of a monodomain class II cyclase, merosterolic acid synthase (MstE). With a minimalistic beta-domain architecture, this cyanobacterial enzyme is able to construct four rings in cytotoxic meroterpenoids with a sterol-like topology. The structures with bound substrate, product, and inhibitor provide detailed snapshots of a cyclization mechanism largely governed by residues located in a noncanonical enzyme region. Our results complement the few known class II cyclase crystal structures, while also indicating that archaic monodomain cyclases might have already catalyzed complex reaction cascades. A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.,Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||