1pp9: Difference between revisions

Revision as of 05:19, 3 May 2008

Bovine cytochrome bc1 complex with stigmatellin bound

OverviewOverview

Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex. Structure-activity relationship studies have shown that the N-formylamino-salicyl-amide group is responsible for most of the binding specificity, and suggested that a low pKa for the phenolic OH group and an intramolecular H-bond between that OH and the carbonyl O of the salicylamide linkage are important. Two previous X-ray structures of antimycin bound to vertebrate bc1 complex gave conflicting results. A new structure reported here of the bovine mitochondrial bc1 complex at 2.28 A resolution with antimycin bound, allows us for the first time to reliably describe the binding of antimycin and shows that the intramolecular hydrogen bond described in solution and in the small-molecule structure is replaced by one involving the NH rather than carbonyl O of the amide linkage, with rotation of the amide group relative to the aromatic ring. The phenolic OH and formylamino N form H-bonds with conserved Asp228 of cytochrome b, and the formylamino O H-bonds via a water molecule to Lys227. A strong density, the right size and shape for a diatomic molecule is found between the other side of the dilactone ring and the alphaA helix.

About this StructureAbout this Structure

1PP9 is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern., Huang LS, Cobessi D, Tung EY, Berry EA, J Mol Biol. 2005 Aug 19;351(3):573-97. PMID:16024040 Page seeded by OCA on Sat May 3 05:19:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1pp9.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1pp9 |SIZE=350|CAPTION= <scene name='initialview01'>1pp9</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1pp9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=BHG:2-HEXYLOXY-6-HYDROXYMETHYL-TETRAHYDRO-PYRAN-3,4,5-TRIOL'>BHG</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEE:PHOSPHATIDYLETHANOLAMINE'>PEE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene>, <scene name='pdbligand=UQ:UBIQUINONE-10'>UQ</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1pp9|  PDB=1pp9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pp9 OCA], [http://www.ebi.ac.uk/pdbsum/1pp9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pp9 RCSB]</span>
-}}
 '''Bovine cytochrome bc1 complex with stigmatellin bound'''
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 [[Category: Huang, L S.]]
 [[Category: Tung, E Y.]]
-[[Category: complex iii]]
+[[Category: Complex iii]]
-[[Category: cytochrome b]]
+[[Category: Cytochrome b]]
-[[Category: cytochrome bc1]]
+[[Category: Cytochrome bc1]]
-[[Category: cytochrome c1]]
+[[Category: Cytochrome c1]]
-[[Category: heme protein]]
+[[Category: Heme protein]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: mitochondrial processing protease]]
+[[Category: Mitochondrial processing protease]]
-[[Category: mpp ubiquinone]]
+[[Category: Mpp ubiquinone]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: redox enzyme]]
+[[Category: Redox enzyme]]
-[[Category: respiratory chain]]
+[[Category: Respiratory chain]]
-[[Category: rieske iron sulfur protein]]
+[[Category: Rieske iron sulfur protein]]
-[[Category: stigmatellin]]
+[[Category: Stigmatellin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:19:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:02:40 2008''