1poo: Difference between revisions

Revision as of 05:18, 3 May 2008

THERMOSTABLE PHYTASE FROM BACILLUS SP

OverviewOverview

Phytases hydrolyze phytic acid to less phosphorylated myo-inositol derivatives and inorganic phosphate. A thermostable phytase is of great value in applications for improving phosphate and metal ion availability in animal feed, and thereby reducing phosphate pollution to the environment. Here, we report a new folding architecture of a six-bladed propeller for phosphatase activity revealed by the 2.1 A crystal structures of a novel, thermostable phytase determined in both the partially and fully Ca2+-loaded states. Binding of two calcium ions to high-affinity calcium binding sites results in a dramatic increase in thermostability (by as much as approximately 30 degrees C in melting temperature) by joining loop segments remote in the amino acid sequence. Binding of three additional calcium ions to low-affinity calcium binding sites at the top of the molecule turns on the catalytic activity of the enzyme by converting the highly negatively charged cleft into a favorable environment for the binding of phytate.

About this StructureAbout this Structure

1POO is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states., Ha NC, Oh BC, Shin S, Kim HJ, Oh TK, Kim YO, Choi KY, Oh BH, Nat Struct Biol. 2000 Feb;7(2):147-53. PMID:10655618 Page seeded by OCA on Sat May 3 05:18:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1poo.gif|left|200px]]
- {{Structure
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-|PDB= 1poo |SIZE=350|CAPTION= <scene name='initialview01'>1poo</scene>, resolution 2.1&Aring;
+The line below this paragraph, containing "STRUCTURE_1poo", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PHY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])
+-->
-|DOMAIN=
+{{STRUCTURE_1poo|  PDB=1poo  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1poo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1poo OCA], [http://www.ebi.ac.uk/pdbsum/1poo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1poo RCSB]</span>
-}}
 '''THERMOSTABLE PHYTASE FROM BACILLUS SP'''
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 [[Category: Ha, N C.]]
 [[Category: Oh, B H.]]
-[[Category: bacillus]]
+[[Category: Bacillus]]
-[[Category: phosphatase]]
+[[Category: Phosphatase]]
-[[Category: phytasephytase]]
+[[Category: Phytasephytase]]
-[[Category: thermostable]]
+[[Category: Thermostable]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:18:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:02:26 2008''