6qaz: Difference between revisions
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<StructureSection load='6qaz' size='340' side='right'caption='[[6qaz]], [[Resolution|resolution]] 1.02Å' scene=''> | <StructureSection load='6qaz' size='340' side='right'caption='[[6qaz]], [[Resolution|resolution]] 1.02Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6qaz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[6qaz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QAZ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.02Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qaz OCA], [https://pdbe.org/6qaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qaz RCSB], [https://www.ebi.ac.uk/pdbsum/6qaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qaz ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qaz OCA], [https://pdbe.org/6qaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qaz RCSB], [https://www.ebi.ac.uk/pdbsum/6qaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qaz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Beyer | [[Category: Metagenome]] | ||
[[Category: Iwai | [[Category: Beyer HM]] | ||
[[Category: Li | [[Category: Iwai H]] | ||
[[Category: Mikula | [[Category: Li M]] | ||
[[Category: Wlodawer | [[Category: Mikula KM]] | ||
[[Category: Wlodawer A]] | |||
Latest revision as of 14:56, 24 January 2024
Crystal structure of gp41-1 inteinCrystal structure of gp41-1 intein
Structural highlights
Publication Abstract from PubMedProtein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 A-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein (HINT) superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins. The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.,Michael Beyer H, Malgorzata Mikula K, Li M, Wlodawer A, Iwai H FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. PMID:31665813[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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