1pjm: Difference between revisions

Revision as of 05:09, 3 May 2008

Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex

OverviewOverview

Importin-alpha is the nuclear import receptor that recognizes cargo proteins carrying conventional basic monopartite and bipartite nuclear localization sequences (NLSs) and facilitates their transport into the nucleus. Bipartite NLSs contain two clusters of basic residues, connected by linkers of variable lengths. To determine the structural basis of the recognition of diverse bipartite NLSs by mammalian importin-alpha, we co-crystallized a non-autoinhibited mouse receptor protein with peptides corresponding to the NLSs from human retinoblastoma protein and Xenopus laevis phosphoprotein N1N2, containing diverse sequences and lengths of the linker. We show that the basic clusters interact analogously in both NLSs, but the linker sequences adopt different conformations, whereas both make specific contacts with the receptor. The available data allow us to draw general conclusions about the specificity of NLS binding by importin-alpha and facilitate an improved definition of the consensus sequence of a conventional basic/bipartite NLS (KRX10-12KRRK) that can be used to identify novel nuclear proteins.

About this StructureAbout this Structure

1PJM is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha., Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B, J Biol Chem. 2003 Jul 25;278(30):27981-7. Epub 2003 Apr 14. PMID:12695505 Page seeded by OCA on Sat May 3 05:09:30 2008

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OCA

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 [[Image:1pjm.gif|left|200px]]
- {{Structure
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-|GENE= KPNA2 OR RCH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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-|RELATEDENTRY=[[1ejl|1EJL]], [[1ejy|1EJY]], [[1iq1|1IQ1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjm OCA], [http://www.ebi.ac.uk/pdbsum/1pjm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pjm RCSB]</span>
-}}
 '''Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex'''
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 [[Category: Kobe, B.]]
 [[Category: Teh, T.]]
-[[Category: bipartite nl]]
+[[Category: Bipartite nl]]
-[[Category: human retinoblastoma protein]]
+[[Category: Human retinoblastoma protein]]
-[[Category: importin alpha/karyopherin alpha]]
+[[Category: Importin alpha/karyopherin alpha]]
-[[Category: nuclear localization sequence (nls) recognition]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:09:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:00:33 2008''