5pd2: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 58)== | ==PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 58)== | ||
<StructureSection load='5pd2' size='340' side='right' caption='[[5pd2]], [[Resolution|resolution]] 1.79Å' scene=''> | <StructureSection load='5pd2' size='340' side='right'caption='[[5pd2]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5pd2]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5pd2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PD2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5pd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5pd2 OCA], [https://pdbe.org/5pd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5pd2 RCSB], [https://www.ebi.ac.uk/pdbsum/5pd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5pd2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/BAZ2B_HUMAN BAZ2B_HUMAN] May play a role in transcriptional regulation interacting with ISWI. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 19: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5pd2" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5pd2" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Bromodomain adjacent to zinc finger 3D structures|Bromodomain adjacent to zinc finger 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Arrowsmith | [[Category: Large Structures]] | ||
[[Category: Bountra | [[Category: Arrowsmith CH]] | ||
[[Category: Bradley | [[Category: Bountra C]] | ||
[[Category: Brandao-Neto | [[Category: Bradley AR]] | ||
[[Category: Brennan | [[Category: Brandao-Neto J]] | ||
[[Category: Collins | [[Category: Brennan PE]] | ||
[[Category: Cox | [[Category: Collins P]] | ||
[[Category: Cox O]] | |||
[[Category: Dias | [[Category: Dias A]] | ||
[[Category: Douangamath | [[Category: Douangamath A]] | ||
[[Category: Edwards | [[Category: Edwards A]] | ||
[[Category: Fairhead | [[Category: Fairhead M]] | ||
[[Category: Krojer | [[Category: Krojer T]] | ||
[[Category: MacLean | [[Category: MacLean E]] | ||
[[Category: Ng | [[Category: Ng J]] | ||
[[Category: Pearce | [[Category: Pearce NM]] | ||
[[Category: Renjie | [[Category: Renjie Z]] | ||
[[Category: Sethi | [[Category: Sethi R]] | ||
[[Category: Talon | [[Category: Talon R]] | ||
[[Category: Vollmar | [[Category: Vollmar M]] | ||
[[Category: Wright | [[Category: Wright N]] | ||
[[Category: | [[Category: Von Delft F]] | ||
Revision as of 04:41, 28 December 2023
PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 58)PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 58)
Structural highlights
FunctionBAZ2B_HUMAN May play a role in transcriptional regulation interacting with ISWI. Publication Abstract from PubMedIn macromolecular crystallography, the rigorous detection of changed states (for example, ligand binding) is difficult unless signal is strong. Ambiguous ('weak' or 'noisy') density is experimentally common, since molecular states are generally only fractionally present in the crystal. Existing methodologies focus on generating maximally accurate maps whereby minor states become discernible; in practice, such map interpretation is disappointingly subjective, time-consuming and methodologically unsound. Here we report the PanDDA method, which automatically reveals clear electron density for the changed state-even from inaccurate maps-by subtracting a proportion of the confounding 'ground state'; changed states are objectively identified from statistical analysis of density distributions. The method is completely general, implying new best practice for all changed-state studies, including the routine collection of multiple ground-state crystals. More generally, these results demonstrate: the incompleteness of atomic models; that single data sets contain insufficient information to model them fully; and that accuracy requires further map-deconvolution approaches. A multi-crystal method for extracting obscured crystallographic states from conventionally uninterpretable electron density.,Pearce NM, Krojer T, Bradley AR, Collins P, Nowak RP, Talon R, Marsden BD, Kelm S, Shi J, Deane CM, von Delft F Nat Commun. 2017 Apr 24;8:15123. doi: 10.1038/ncomms15123. PMID:28436492[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||