1pdn: Difference between revisions

Revision as of 04:58, 3 May 2008

CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS

OverviewOverview

The 2.5 A resolution structure of a cocrystal containing the paired domain from the Drosophila paired (prd) protein and a 15 bp site shows structurally independent N-terminal and C-terminal subdomains. Each of these domains contains a helical region resembling the homeodomain and the Hin recombinase. The N-terminal domain makes extensive DNA contacts, using a novel beta turn motif that binds in the minor groove and a helix-turn-helix unit with a docking arrangement surprisingly similar to that of the lambda repressor. The C-terminal domain is not essential for prd binding and does not contact the optimized site. All known developmental missense mutations in the paired box of mammalian Pax genes map to the N-terminal subdomain, and most of them are found at the protein-DNA interface.

About this StructureAbout this Structure

1PDN is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a paired domain-DNA complex at 2.5 A resolution reveals structural basis for Pax developmental mutations., Xu W, Rould MA, Jun S, Desplan C, Pabo CO, Cell. 1995 Feb 24;80(4):639-50. PMID:7867071 Page seeded by OCA on Sat May 3 04:58:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1pdn.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1pdn |SIZE=350|CAPTION= <scene name='initialview01'>1pdn</scene>, resolution 2.500&Aring;
+The line below this paragraph, containing "STRUCTURE_1pdn", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= PRD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
+-->
-|DOMAIN=
+{{STRUCTURE_1pdn|  PDB=1pdn  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pdn OCA], [http://www.ebi.ac.uk/pdbsum/1pdn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pdn RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS'''
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 [[Category: Rould, M A.]]
 [[Category: Xu, W.]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: double helix]]
+[[Category: Double helix]]
-[[Category: paired domain]]
+[[Category: Paired domain]]
-[[Category: pax]]
+[[Category: Pax]]
-[[Category: prd]]
+[[Category: Prd]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:58:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:58:17 2008''