1pc9: Difference between revisions

Revision as of 04:55, 3 May 2008

Crystal Structure of BnSP-6, a Lys49-Phospholipase A2

OverviewOverview

Phospholipases A(2) are components of Bothrops venoms responsible for disruption of cell membrane integrity via hydrolysis of its phospholipids. A class of PLA(2)-like proteins has been described which despite PLA(2) activity on artificial substrate, due to a D49K mutation, is still highly myonecrotic. This work reports the X-ray structure determination of two Lys49-PLA(2)s from Bothrops neuwiedi pauloensis (BnSP-7 and BnSP-6) and, for the first time, the comparison of eight dimeric Lys49-PLA(2)s. This comparison reveals that there are not just two ("open" and "closed") but at least six different conformations. The binding of fatty acid observed in three recent Lys49-PLA(2) structures seems to be independent of their quaternary conformation. Cys29 polarization by Lys122 is not significant for BnSP-7 and BnSP-6 or other structures not bound by fatty acids. These structures may be in an "active" state when nothing is bound to them and the Lys122/Cys29 interactions are weak or absent.

About this StructureAbout this Structure

1PC9 is a Single protein structure of sequence from Bothrops neuwiedi pauloensis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:14623331 Page seeded by OCA on Sat May 3 04:55:53 2008

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OCA

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 [[Image:1pc9.gif|left|200px]]
- {{Structure
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span>
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-|RELATEDENTRY=[[1pa0|1PA0]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc9 OCA], [http://www.ebi.ac.uk/pdbsum/1pc9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pc9 RCSB]</span>
-}}
 '''Crystal Structure of BnSP-6, a Lys49-Phospholipase A2'''
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 Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623331 14623331]
 [[Category: Bothrops neuwiedi pauloensis]]
-[[Category: Phospholipase A(2)]]
 [[Category: Single protein]]
 [[Category: Fontes, M R.M.]]
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 [[Category: Magro, A J.]]
 [[Category: Soares, A M.]]
-[[Category: bothrop]]
+[[Category: Bothrop]]
-[[Category: crystal structure]]
+[[Category: Crystal structure]]
-[[Category: lys49-phospholipase a2]]
+[[Category: Lys49-phospholipase a2]]
-[[Category: myotoxin]]
+[[Category: Myotoxin]]
-[[Category: venom.]]
+[[Category: Venom.]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:55:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:57:53 2008''