4u55: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4u55]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukk 4ukk], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukl 4ukl], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukm 4ukm], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukn 4ukn] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4uko 4uko]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U55 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4u55]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukk 4ukk], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukl 4ukl], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukm 4ukm], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ukn 4ukn] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4uko 4uko]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U55 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3K8:(14AR)-2,3,6-TRIMETHOXY-11,12,13,14,14A,15-HEXAHYDRO-9H-DIBENZO[F,H]PYRIDO[1,2-B]ISOQUINOLINE'>3K8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OHX:OSMIUM+(III)+HEXAMMINE'>OHX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3K8:(14AR)-2,3,6-TRIMETHOXY-11,12,13,14,14A,15-HEXAHYDRO-9H-DIBENZO[F,H]PYRIDO[1,2-B]ISOQUINOLINE'>3K8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OHX:OSMIUM+(III)+HEXAMMINE'>OHX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u55 OCA], [https://pdbe.org/4u55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u55 RCSB], [https://www.ebi.ac.uk/pdbsum/4u55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u55 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u55 OCA], [https://pdbe.org/4u55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u55 RCSB], [https://www.ebi.ac.uk/pdbsum/4u55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u55 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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==See Also== | ==See Also== | ||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]] | *[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]] | ||
== References == | == References == | ||
Latest revision as of 15:26, 20 December 2023
Crystal structure of Cryptopleurine bound to the yeast 80S ribosomeCrystal structure of Cryptopleurine bound to the yeast 80S ribosome
Structural highlights
FunctionPublication Abstract from PubMedThe ribosome is a molecular machine responsible for protein synthesis and a major target for small-molecule inhibitors. Compared to the wealth of structural information available on ribosome-targeting antibiotics in bacteria, our understanding of the binding mode of ribosome inhibitors in eukaryotes is currently limited. Here we used X-ray crystallography to determine 16 high-resolution structures of 80S ribosomes from Saccharomyces cerevisiae in complexes with 12 eukaryote-specific and 4 broad-spectrum inhibitors. All inhibitors were found associated with messenger RNA and transfer RNA binding sites. In combination with kinetic experiments, the structures suggest a model for the action of cycloheximide and lactimidomycin, which explains why lactimidomycin, the larger compound, specifically targets the first elongation cycle. The study defines common principles of targeting and resistance, provides insights into translation inhibitor mode of action and reveals the structural determinants responsible for species selectivity which could guide future drug development. Structural basis for the inhibition of the eukaryotic ribosome.,Garreau de Loubresse N, Prokhorova I, Holtkamp W, Rodnina MV, Yusupova G, Yusupov M Nature. 2014 Sep 25;513(7519):517-22. doi: 10.1038/nature13737. Epub 2014 Sep 10. PMID:25209664[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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