4bb3: Difference between revisions
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<StructureSection load='4bb3' size='340' side='right'caption='[[4bb3]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='4bb3' size='340' side='right'caption='[[4bb3]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4bb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4bb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BB3 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=KKA:(2S)-2-AZANYL-6-OXIDANYLIDENE-6-[[(2S)-1-OXIDANYL-1-OXIDANYLIDENE-4-SULFANYL-BUTAN-2-YL]AMINO]HEXANOIC+ACID'>KKA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=KKA:(2S)-2-AZANYL-6-OXIDANYLIDENE-6-[[(2S)-1-OXIDANYL-1-OXIDANYLIDENE-4-SULFANYL-BUTAN-2-YL]AMINO]HEXANOIC+ACID'>KKA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bb3 OCA], [https://pdbe.org/4bb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bb3 RCSB], [https://www.ebi.ac.uk/pdbsum/4bb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bb3 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bb3 OCA], [https://pdbe.org/4bb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bb3 RCSB], [https://www.ebi.ac.uk/pdbsum/4bb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bb3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/IPNA_EMENI IPNA_EMENI] Isopenicillin N synthase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic (PubMed:3319778, PubMed:11755401). IpnA catalyzes the cyclization of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) to form isopenicillin N (IPN) that contains the beta-lactam nucleus (PubMed:3319778, PubMed:11755401, PubMed:28703303). The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS acvA. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase penDE to yield penicillin in the peroxisomal matrix (By similarity).[UniProtKB:P08703]<ref>PMID:11755401</ref> <ref>PMID:28703303</ref> <ref>PMID:3319778</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aspergillus nidulans | [[Category: Aspergillus nidulans FGSC A4]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Clifton | [[Category: Clifton IJ]] | ||
[[Category: Daruzzaman | [[Category: Daruzzaman A]] | ||
[[Category: Rutledge | [[Category: Rutledge PJ]] | ||