4ag5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='4ag5' size='340' side='right'caption='[[4ag5]], [[Resolution|resolution]] 2.45Å' scene=''> | <StructureSection load='4ag5' size='340' side='right'caption='[[4ag5]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ag5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4ag5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_pseudethanolicus Thermoanaerobacter pseudethanolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AG5 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ag5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ag5 OCA], [https://pdbe.org/4ag5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ag5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ag5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ag5 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ag5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ag5 OCA], [https://pdbe.org/4ag5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ag5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ag5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ag5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B0KAW2_THEP3 B0KAW2_THEP3] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 21: | Line 23: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Lian | [[Category: Thermoanaerobacter pseudethanolicus]] | ||
[[Category: Orlova | [[Category: Lian PW]] | ||
[[Category: Thalassinos | [[Category: Orlova EV]] | ||
[[Category: Waksman | [[Category: Thalassinos K]] | ||
[[Category: Wallden | [[Category: Waksman G]] | ||
[[Category: Wang | [[Category: Wallden K]] | ||
[[Category: Williams | [[Category: Wang L]] | ||
[[Category: Yan | [[Category: Williams R]] | ||
[[Category: Yan J]] | |||
Latest revision as of 14:26, 20 December 2023
Structure of VirB4 of Thermoanaerobacter pseudethanolicusStructure of VirB4 of Thermoanaerobacter pseudethanolicus
Structural highlights
FunctionPublication Abstract from PubMedType IV secretion (T4S) systems mediate the transfer of proteins and DNA across the cell envelope of bacteria. These systems play important roles in bacterial pathogenesis and in horizontal transfer of antibiotic resistance. The VirB4 ATPase of the T4S system is essential for both the assembly of the system and substrate transfer. In this article, we present the crystal structure of the C-terminal domain of Thermoanaerobacter pseudethanolicus VirB4. This structure is strikingly similar to that of another T4S ATPase, VirD4, a protein that shares only 12% sequence identity with VirB4. The VirB4 domain purifies as a monomer, but the full-length protein is observed in a monomer-dimer equilibrium, even in the presence of nucleotides and DNAs. We also report the negative stain electron microscopy structure of the core complex of the T4S system of the Escherichia coli pKM101 plasmid, with VirB4 bound. In this structure, VirB4 is also monomeric and bound through its N-terminal domain to the core's VirB9 protein. Remarkably, VirB4 is observed bound to the side of the complex where it is ideally placed to play its known regulatory role in substrate transfer. Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system.,Wallden K, Williams R, Yan J, Lian PW, Wang L, Thalassinos K, Orlova EV, Waksman G Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11348-53. Epub 2012 Jun 27. PMID:22745169[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||