1oye: Difference between revisions

Revision as of 04:25, 3 May 2008

Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump

OverviewOverview

Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.

About this StructureAbout this Structure

1OYE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump., Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr, Science. 2003 May 9;300(5621):976-80. PMID:12738864 Page seeded by OCA on Sat May 3 04:25:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1oye.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1oye |SIZE=350|CAPTION= <scene name='initialview01'>1oye</scene>, resolution 3.48&Aring;
+The line below this paragraph, containing "STRUCTURE_1oye", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CPF:1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC+ACID'>CPF</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= ACRB OR ACRE OR B0462 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1oye|  PDB=1oye  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oye OCA], [http://www.ebi.ac.uk/pdbsum/1oye PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oye RCSB]</span>
-}}
 '''Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump'''
@@ Line 30: / Line 27: @@
 [[Category: Yu, E W.]]
 [[Category: Zgurskaya, H I.]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:25:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:08 2008''