1oyb: Difference between revisions

Revision as of 04:25, 3 May 2008

OLD YELLOW ENZYME AT 2 ANGSTROMS RESOLUTION: OVERALL STRUCTURE, LIGAND BINDING AND COMPARISON WITH RELATED FLAVOPROTEINS

OverviewOverview

BACKGROUND: Old yellow enzyme (OYE) was the first flavoenzyme purified, but its function is still unknown. Nevertheless, the NADPH oxidase activity, the flavin mononucleotide environment and the ligand-binding properties of OYE have been extensively studied by biochemical and spectroscopic approaches. Full interpretation of these data requires structural information. RESULTS: The crystal structures of oxidized and reduced OYE at 2 A resolution reveal an alpha/beta-barrel topology clearly related to trimethylamine dehydrogenase. Complexes of OYE with p-hydroxybenzaldehyde, beta-estradiol, and an NADPH analog show all three binding at a common site, stacked on the flavin. The putative NADPH binding mode is novel as it involves primary recognition of the nicotinamide mononucleotide portion. CONCLUSIONS: This work shows that the striking spectral changes seen upon phenol binding are due to close physical association of the flavin and phenolate. It also identifies the structural class of OYE and suggests that if NADPH is its true substrate, then OYE has adopted NADPH dependence during evolution.

About this StructureAbout this Structure

1OYB is a Single protein structure of sequence from Saccharomyces pastorianus. Full crystallographic information is available from OCA.

ReferenceReference

Old yellow enzyme at 2 A resolution: overall structure, ligand binding, and comparison with related flavoproteins., Fox KM, Karplus PA, Structure. 1994 Nov 15;2(11):1089-105. PMID:7881908 Page seeded by OCA on Sat May 3 04:25:51 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1oyb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1oyb |SIZE=350|CAPTION= <scene name='initialview01'>1oyb</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1oyb", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HBA:P-HYDROXYBENZALDEHYDE'>HBA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=27292 Saccharomyces pastorianus])
+-->
-|DOMAIN=
+{{STRUCTURE_1oyb|  PDB=1oyb  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oyb OCA], [http://www.ebi.ac.uk/pdbsum/1oyb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oyb RCSB]</span>
-}}
 '''OLD YELLOW ENZYME AT 2 ANGSTROMS RESOLUTION: OVERALL STRUCTURE, LIGAND BINDING AND COMPARISON WITH RELATED FLAVOPROTEINS'''
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 [[Category: Fox, K M.]]
 [[Category: Karplus, P A.]]
-[[Category: oxidoreductase(flavoprotein)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:25:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:09 2008''