2j6r: Difference between revisions
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<StructureSection load='2j6r' size='340' side='right'caption='[[2j6r]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='2j6r' size='340' side='right'caption='[[2j6r]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2j6r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2j6r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J6R FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6r OCA], [https://pdbe.org/2j6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j6r RCSB], [https://www.ebi.ac.uk/pdbsum/2j6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6r ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6r OCA], [https://pdbe.org/2j6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j6r RCSB], [https://www.ebi.ac.uk/pdbsum/2j6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/FAEG2_ECOLX FAEG2_ECOLX] K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bouckaert | [[Category: Bouckaert J]] | ||
[[Category: Buts | [[Category: Buts L]] | ||
[[Category: Greve | [[Category: De Greve H]] | ||
[[Category: Joensuu | [[Category: Joensuu JJ]] | ||
[[Category: Kotiaho | [[Category: Kotiaho M]] | ||
[[Category: Niklander-Teeri V]] | |||
[[Category: Niklander-Teeri | [[Category: Panjikar S]] | ||
[[Category: Panjikar | [[Category: Van Molle I]] | ||
[[Category: | [[Category: Wyns L]] | ||
[[Category: | |||
Latest revision as of 17:36, 13 December 2023
FaeG from F4ac ETEC strain GIS26, produced in tobacco plant chloroplastFaeG from F4ac ETEC strain GIS26, produced in tobacco plant chloroplast
Structural highlights
FunctionFAEG2_ECOLX K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedF4 fimbriae encoded by the fae operon are the major colonization factors associated with porcine neonatal and postweaning diarrhoea caused by enterotoxigenic Escherichia coli (ETEC). Via the chaperone/usher pathway, the F4 fimbriae are assembled as long polymers of the major subunit FaeG, which also possesses the adhesive properties of the fimbriae. Intrinsically, the incomplete fold of fimbrial subunits renders them unstable and susceptible to aggregation and/or proteolytic degradation in the absence of a specific periplasmic chaperone. In order to test the possibility of producing FaeG in plants, FaeG expression was studied in transgenic tobacco plants. FaeG was directed to different subcellular compartments by specific targeting signals. Targeting of FaeG to the chloroplast results in much higher yields than FaeG targeting to the endoplasmic reticulum or the apoplast. Two chloroplast-targeted FaeG variants were purified from tobacco plants and crystallized. The crystal structures show that chloroplasts circumvent the absence of the fimbrial assembly machinery by assembling FaeG into strand-swapped dimers. Furthermore, the structures reveal how FaeG combines the structural requirements of a major fimbrial subunit with its adhesive role by grafting an additional domain on its Ig-like core. Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers.,Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22. PMID:17368480[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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