1ox7: Difference between revisions

Revision as of 04:23, 3 May 2008

Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound

OverviewOverview

Cytosine deaminase (CD) catalyzes the deamination of cytosine and is only present in prokaryotes and fungi, where it is a member of the pyrimidine salvage pathway. The enzyme is of interest both for antimicrobial drug design and gene therapy applications against tumors. The structure of Saccharomyces cerevisiae CD has been determined in the presence and absence of a mechanism-based inhibitor, at 1.14 and 1.43 A resolution, respectively. The enzyme forms an alpha/beta fold similar to bacterial cytidine deaminase, but with no similarity to the alpha/beta barrel fold used by bacterial cytosine deaminase or mammalian adenosine deaminase. The structures observed for bacterial, fungal, and mammalian nucleic acid deaminases represent an example of the parallel evolution of two unique protein folds to carry out the same reaction on a diverse array of substrates.

About this StructureAbout this Structure

1OX7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy., Ireton GC, Black ME, Stoddard BL, Structure. 2003 Aug;11(8):961-72. PMID:12906827 Page seeded by OCA on Sat May 3 04:23:24 2008

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OCA

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 [[Image:1ox7.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ox7", creates the "Structure Box" on the page.
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] </span>
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-|GENE= FCY1 OR YPR062W OR YP9499.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ox7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ox7 OCA], [http://www.ebi.ac.uk/pdbsum/1ox7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ox7 RCSB]</span>
-}}
 '''Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound'''
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 [[Category: Ireton, G C.]]
 [[Category: Stoddard, B L.]]
-[[Category: aminohydrolase]]
+[[Category: Aminohydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:23:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:36 2008''