2co7: Difference between revisions
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<StructureSection load='2co7' size='340' side='right'caption='[[2co7]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='2co7' size='340' side='right'caption='[[2co7]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2co7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2co7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CO7 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2co7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2co7 OCA], [https://pdbe.org/2co7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2co7 RCSB], [https://www.ebi.ac.uk/pdbsum/2co7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2co7 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2co7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2co7 OCA], [https://pdbe.org/2co7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2co7 RCSB], [https://www.ebi.ac.uk/pdbsum/2co7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2co7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</div> | </div> | ||
<div class="pdbe-citations 2co7" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2co7" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Pilin 3D structures|Pilin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] | ||
[[Category: Ashcroft | [[Category: Ashcroft AE]] | ||
[[Category: Hannan | [[Category: Hannan TJ]] | ||
[[Category: Hultgren | [[Category: Hultgren SJ]] | ||
[[Category: Radford | [[Category: Radford SE]] | ||
[[Category: Remaut | [[Category: Remaut H]] | ||
[[Category: Rose | [[Category: Rose RJ]] | ||
[[Category: Waksman | [[Category: Waksman G]] | ||
Revision as of 17:24, 13 December 2023
Salmonella enterica SafA pilin in complex with the SafB chaperone (Type II)Salmonella enterica SafA pilin in complex with the SafB chaperone (Type II)
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedGram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled. Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism.,Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G Mol Cell. 2006 Jun 23;22(6):831-42. PMID:16793551[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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