2cjw: Difference between revisions
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<StructureSection load='2cjw' size='340' side='right'caption='[[2cjw]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2cjw' size='340' side='right'caption='[[2cjw]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2cjw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2cjw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjw OCA], [https://pdbe.org/2cjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cjw RCSB], [https://www.ebi.ac.uk/pdbsum/2cjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cjw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjw OCA], [https://pdbe.org/2cjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cjw RCSB], [https://www.ebi.ac.uk/pdbsum/2cjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cjw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/GEM_HUMAN GEM_HUMAN] Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cabanie | [[Category: Cabanie L]] | ||
[[Category: Cicolari | [[Category: Cicolari J]] | ||
[[Category: | [[Category: El Marjou A]] | ||
[[Category: Hamoudi | [[Category: Hamoudi F]] | ||
[[Category: Houdusse | [[Category: Houdusse A]] | ||
[[Category: Menetrey J]] | |||
[[Category: Menetrey | [[Category: Perderiset M]] | ||
[[Category: Perderiset | [[Category: Splingard A]] | ||
[[Category: Splingard | [[Category: Wells A]] | ||
[[Category: Wells | [[Category: De Gunzburg J]] | ||
[[Category: | |||
Revision as of 17:19, 13 December 2023
Crystal structure of the small GTPase Gem (GemDNDCaM) in complex to Mg.GDPCrystal structure of the small GTPase Gem (GemDNDCaM) in complex to Mg.GDP
Structural highlights
FunctionGEM_HUMAN Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRGK proteins, encompassing Rad, Gem, Rem1, and Rem2, constitute an intriguing branch of the Ras superfamily; their expression is regulated at the transcription level, they exhibit atypical nucleotide binding motifs, and they carry both large N- and C-terminal extensions. Biochemical and structural studies are required to better understand how such proteins function. Here, we report the first structure for a RGK protein: the crystal structure of a truncated form of the human Gem protein (G domain plus the first part of the C-terminal extension) in complex with Mg.GDP at 2.1 A resolution. It reveals that the G-domain fold and Mg.GDP binding site of Gem are similar to those found for other Ras family GTPases. The first part of the C-terminal extension adopts an alpha-helical conformation that extends along the alpha5 helix and interacts with the tip of the interswitch. Biochemical studies show that the affinities of Gem for GDP and GTP are considerably lower (micromolar range) compared with H-Ras, independent of the presence or absence of N- and C-terminal extensions, whereas its GTPase activity is higher than that of H-Ras and regulated by both extensions. We show how the bulky DXWEX motif, characteristic of the switch II of RGK proteins, affects the conformation of switch I and the phosphate-binding site. Altogether, our data reveal that Gem is a bona fide GTPase that exhibits striking structural and biochemical features that should impact its regulation and cellular activities. Biochemical and structural characterization of the gem GTPase.,Splingard A, Menetrey J, Perderiset M, Cicolari J, Regazzoni K, Hamoudi F, Cabanie L, El Marjou A, Wells A, Houdusse A, de Gunzburg J J Biol Chem. 2007 Jan 19;282(3):1905-15. Epub 2006 Nov 15. PMID:17107948[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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