2bs1: Difference between revisions
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<StructureSection load='2bs1' size='340' side='right'caption='[[2bs1]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='2bs1' size='340' side='right'caption='[[2bs1]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2bs1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2bs1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS1 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs1 OCA], [https://pdbe.org/2bs1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs1 OCA], [https://pdbe.org/2bs1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia phage MS2]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Adams | [[Category: Adams CJ]] | ||
[[Category: Baron | [[Category: Baron AJ]] | ||
[[Category: Grahn | [[Category: Grahn E]] | ||
[[Category: Helgstrand | [[Category: Helgstrand C]] | ||
[[Category: Horn | [[Category: Horn WT]] | ||
[[Category: Lago | [[Category: Lago H]] | ||
[[Category: Liljas | [[Category: Liljas L]] | ||
[[Category: Peabody | [[Category: Peabody DS]] | ||
[[Category: Phillips | [[Category: Phillips SEV]] | ||
[[Category: Stockley | [[Category: Stockley PG]] | ||
[[Category: Stonehouse | [[Category: Stonehouse NJ]] | ||
[[Category: Tars | [[Category: Tars K]] | ||
Latest revision as of 16:54, 13 December 2023
MS2 (N87AE89K mutant) - Qbeta RNA hairpin complexMS2 (N87AE89K mutant) - Qbeta RNA hairpin complex
Structural highlights
FunctionCAPSD_BPMS2 Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.[1] [2] [3] [4] [5] [6] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism. Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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