1otn: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1otn.jpg|left|200px]] | [[Image:1otn.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1otn", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1otn| PDB=1otn | SCENE= }} | |||
}} | |||
'''Calcium-binding mutant of the Internalin B LRR domain''' | '''Calcium-binding mutant of the Internalin B LRR domain''' | ||
| Line 32: | Line 29: | ||
[[Category: Ghosh, P.]] | [[Category: Ghosh, P.]] | ||
[[Category: Marino, M.]] | [[Category: Marino, M.]] | ||
[[Category: | [[Category: Calcium-binding]] | ||
[[Category: | [[Category: Inlb]] | ||
[[Category: | [[Category: Internalin]] | ||
[[Category: | [[Category: Invasion]] | ||
[[Category: | [[Category: Listeria]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:16:05 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 04:16, 3 May 2008
Calcium-binding mutant of the Internalin B LRR domain
OverviewOverview
The Listeria monocytogenes protein InlB promotes invasion of mammalian cells through activation of the receptor tyrosine kinase Met. The InlB N-cap, a approximately 40 residue part of the domain that binds Met, was previously observed to bind two calcium ions in a novel and unusually exposed manner. Because subsequent work raised questions about the existence of these calcium-binding sites, we assayed calcium binding in solution to the InlB N-cap. We show that calcium ions are bound with dissociation constants in the low micromolar range at the two identified sites, and that the sites interact with one another. We demonstrate that the calcium ions are not required for structure, and also find that they have no appreciable effect on Met activation or intracellular invasion. Therefore, our results indicate that the sites are fortuitous in InlB, but also suggest that the simple architecture of the sites may be adaptable for protein engineering purposes.
About this StructureAbout this Structure
1OTN is a Single protein structure of sequence from Listeria monocytogenes. Full crystallographic information is available from OCA.
ReferenceReference
Characterization of the calcium-binding sites of Listeria monocytogenes InlB., Marino M, Banerjee M, Copp J, Dramsi S, Chapman T, van der Geer P, Cossart P, Ghosh P, Biochem Biophys Res Commun. 2004 Apr 2;316(2):379-86. PMID:15020228 Page seeded by OCA on Sat May 3 04:16:05 2008