1ot6: Difference between revisions

Revision as of 04:15, 3 May 2008

CRYOTRAPPED CRYSTAL STRUCTURE OF THE E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN UNDER CONTINUOUS ILLUMINATION AT 110K

OverviewOverview

Eight high-resolution crystal structures of the ground state of photoactive yellow protein (PYP) solved under a variety of conditions reveal that its chromophore is stabilized by two unusually short hydrogen bonds. Both Tyr42 Oeta and Glu46 Oepsilon are separated from the chromophore phenolate oxygen by less than the sum of their atomic van der Waals radii, 2.6 angstroms. This is characteristic of strong hydrogen bonding, in which hydrogen bonds acquire significant covalent character. The hydrogen bond from the protonated Glu46 to the negatively charged phenolate oxygen is 2.58 +/- 0.01 angstroms in length, while that from Tyr42 is considerably shorter, 2.49 +/- 0.01 angstroms. The E46Q mutant was solved to 0.95 angstroms resolution; the isosteric mutation increased the length of the hydrogen bond from Glx46 to the chromophore by 0.29 +/- 0.01 angstroms to that of an average hydrogen bond, 2.88 +/- 0.01 angstroms. The very short hydrogen bond from Tyr42 explains why mutating this residue has such a severe effect on the ground-state structure and PYP photocycle. The effect of isosteric mutations on the photocycle can be largely explained by the alterations to the length and strength of these hydrogen bonds.

About this StructureAbout this Structure

1OT6 is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.

ReferenceReference

Short hydrogen bonds in photoactive yellow protein., Anderson S, Crosson S, Moffat K, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1008-16. Epub 2004, May 21. PMID:15159559 Page seeded by OCA on Sat May 3 04:15:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ot6.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1ot6 |SIZE=350|CAPTION= <scene name='initialview01'>1ot6</scene>, resolution 0.95&Aring;
+The line below this paragraph, containing "STRUCTURE_1ot6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HC4:4&#39;-HYDROXYCINNAMIC+ACID'>HC4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])
+-->
-|DOMAIN=
+{{STRUCTURE_1ot6|  PDB=1ot6  |  SCENE=  }}
-|RELATEDENTRY=[[1ot9|1OT9]], [[1ot6|1OT6]], [[1ota|1OTA]], [[1otb|1OTB]], [[1ot1|1OT1]], [[1ote|1OTE]], [[1otd|1OTD]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot6 OCA], [http://www.ebi.ac.uk/pdbsum/1ot6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ot6 RCSB]</span>
-}}
 '''CRYOTRAPPED CRYSTAL STRUCTURE OF THE E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN UNDER CONTINUOUS ILLUMINATION AT 110K'''
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 [[Category: Crosson, S.]]
 [[Category: Moffat, K.]]
-[[Category: cryotrapping]]
+[[Category: Cryotrapping]]
-[[Category: pyp]]
+[[Category: Pyp]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:15:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:50:01 2008''