1orp: Difference between revisions

Revision as of 04:12, 3 May 2008

Structure of a Trapped Endonuclease III-DNA Covalent Intermediate: Estranged-Adenine Complex

OverviewOverview

Nearly all cells express proteins that confer resistance to the mutagenic effects of oxidative DNA damage. The primary defense against the toxicity of oxidative nucleobase lesions in DNA is the base-excision repair (BER) pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA glycosylase with repair activity specific for oxidized pyrimidine lesions in duplex DNA. We have determined the crystal structure of a trapped intermediate that represents EndoIII frozen in the act of repairing DNA. The structure of the protein-DNA complex provides insight into the ability of EndoIII to recognize and repair a diverse array of oxidatively damaged bases. This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structure of a trapped endonuclease III-DNA covalent intermediate., Fromme JC, Verdine GL, EMBO J. 2003 Jul 1;22(13):3461-71. PMID:12840008 Page seeded by OCA on Sat May 3 04:12:04 2008

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OCA

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 [[Image:1orp.gif|left|200px]]
- {{Structure
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-|GENE= nth ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[2abk|2ABK]], [[1orn|1ORN]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1orp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orp OCA], [http://www.ebi.ac.uk/pdbsum/1orp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1orp RCSB]</span>
-}}
 '''Structure of a Trapped Endonuclease III-DNA Covalent Intermediate: Estranged-Adenine Complex'''
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 ==About this Structure==
-ORP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORP OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORP OCA].
 ==Reference==
 Structure of a trapped endonuclease III-DNA covalent intermediate., Fromme JC, Verdine GL, EMBO J. 2003 Jul 1;22(13):3461-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12840008 12840008]
-[[Category: Geobacillus stearothermophilus]]
-[[Category: Protein complex]]
 [[Category: Fromme, J C.]]
 [[Category: Verdine, G L.]]
-[[Category: [4fe-4s] cluster]]
+[[Category: Dna glycosylase]]
-[[Category: dna glycosylase]]
+[[Category: Dna repair]]
-[[Category: dna repair]]
+[[Category: Iron-sulfur cluster]]
-[[Category: iron-sulfur cluster]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:12:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:20 2008''