7xwm: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7xwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Meyerozyma_guilliermondii Meyerozyma guilliermondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XWM FirstGlance]. <br> | <table><tr><td colspan='2'>[[7xwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Meyerozyma_guilliermondii Meyerozyma guilliermondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XWM FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xwm OCA], [https://pdbe.org/7xwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xwm RCSB], [https://www.ebi.ac.uk/pdbsum/7xwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xwm ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xwm OCA], [https://pdbe.org/7xwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xwm RCSB], [https://www.ebi.ac.uk/pdbsum/7xwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xwm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
Latest revision as of 21:02, 29 November 2023
structure of patulin-detoxifying enzyme Y155F/V187K with NADPHstructure of patulin-detoxifying enzyme Y155F/V187K with NADPH
Structural highlights
FunctionPublication Abstract from PubMedPatulin is a fatal mycotoxin that is widely detected in drinking water and fruit-derived products contaminated by diverse filamentous fungi. CgSDR from Candida guilliermondii represents the first NADPH-dependent short-chain dehydrogenase/reductase that catalyzes the reduction of patulin to the nontoxic E-ascladiol. To elucidate the catalytic mechanism of CgSDR, we solved its crystal structure in complex with cofactor and substrate. Structural analyses indicate that patulin is situated in a hydrophobic pocket adjacent to the cofactor, with the hemiacetal ring orienting toward the nicotinamide moiety of NADPH. In addition, we conducted structure-guided engineering to modify substrate-binding residue V187 and obtained variant V187F, V187K and V187W, whose catalytic activity was elevated by 3.9-, 2.2- and 1.7-fold, respectively. The crystal structures of CgSDR variants suggest that introducing additional aromatic stacking or hydrogen-bonding interactions to bind the lactone ring of patulin might account for the observed enhanced activity. These results illustrate the catalytic mechanism of SDR-mediated patulin detoxification for the first time and provide the upgraded variants that exhibit tremendous potentials in industrial applications. Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin.,Dai L, Li H, Huang JW, Hu Y, He M, Yang Y, Min J, Guo RT, Chen CC Int J Biol Macromol. 2022 Dec 1;222(Pt A):421-428. doi: , 10.1016/j.ijbiomac.2022.09.121. Epub 2022 Sep 20. PMID:36176222[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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