1ond: Difference between revisions

Revision as of 04:03, 3 May 2008

THE CRYSTAL STRUCTURE OF THE 50S LARGE RIBOSOMAL SUBUNIT FROM DEINOCOCCUS RADIODURANS COMPLEXED WITH TROLEANDOMYCIN MACROLIDE ANTIBIOTIC

OverviewOverview

Nascent proteins emerge out of ribosomes through an exit tunnel, which was assumed to be a firmly built passive path. Recent biochemical results, however, indicate that the tunnel plays an active role in sequence-specific gating of nascent chains and in responding to cellular signals. Consistently, modulation of the tunnel shape, caused by the binding of the semi-synthetic macrolide troleandomycin to the large ribosomal subunit from Deinococcus radiodurans, was revealed crystallographically. The results provide insights into the tunnel dynamics at high resolution. Here we show that, in addition to the typical steric blockage of the ribosomal tunnel by macrolides, troleandomycin induces a conformational rearrangement in a wall constituent, protein L22, flipping the tip of its highly conserved beta-hairpin across the tunnel. On the basis of mutations that alleviate elongation arrest, the tunnel motion could be correlated with sequence discrimination and gating, suggesting that specific arrest motifs within nascent chain sequences may induce a similar gating mechanism.

About this StructureAbout this Structure

1OND is a Protein complex structure of sequences from Deinococcus radiodurans. Full crystallographic information is available from OCA.

ReferenceReference

Structural insight into the role of the ribosomal tunnel in cellular regulation., Berisio R, Schluenzen F, Harms J, Bashan A, Auerbach T, Baram D, Yonath A, Nat Struct Biol. 2003 May;10(5):366-70. PMID:12665853 Page seeded by OCA on Sat May 3 04:03:40 2008

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OCA

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 [[Image:1ond.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ond FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ond OCA], [http://www.ebi.ac.uk/pdbsum/1ond PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ond RCSB]</span>
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 '''THE CRYSTAL STRUCTURE OF THE 50S LARGE RIBOSOMAL SUBUNIT FROM DEINOCOCCUS RADIODURANS COMPLEXED WITH TROLEANDOMYCIN MACROLIDE ANTIBIOTIC'''
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 [[Category: Schluenzen, F.]]
 [[Category: Yonath, A.]]
-[[Category: antibiotic]]
+[[Category: Antibiotic]]
-[[Category: blockage]]
+[[Category: Blockage]]
-[[Category: exit-tunnel l22]]
+[[Category: Exit-tunnel l22]]
-[[Category: macrolide]]
+[[Category: Macrolide]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
-[[Category: trna]]
+[[Category: Trna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:03:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:47:42 2008''