7fca: Difference between revisions
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==== | ==PfkB(Mycobacterium marinum)== | ||
<StructureSection load='7fca' size='340' side='right'caption='[[7fca]]' scene=''> | <StructureSection load='7fca' size='340' side='right'caption='[[7fca]], [[Resolution|resolution]] 2.21Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7fca]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_M Mycobacterium marinum M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FCA FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fca OCA], [https://pdbe.org/7fca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fca RCSB], [https://www.ebi.ac.uk/pdbsum/7fca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fca ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fca OCA], [https://pdbe.org/7fca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fca RCSB], [https://www.ebi.ac.uk/pdbsum/7fca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fca ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B2HEF4_MYCMM B2HEF4_MYCMM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphofructokinase B (PfkB) belongs to the ribokinase family, which uses the phosphorylated sugar as substrate, and catalyzes fructose-6-phosphate into fructose-1,6-diphosphate. However, the structural basis of Mycobacterium marinum PfkB is not clear. Here, we found that the PfkB protein was monomeric in solution, which was different from most enzymes in this family. The crystal structure of PfkB protein from M. marinum was solved at a resolution of 2.21 A. The PfkB structure consists of two domains, a major three-layered alpha/beta/alpha sandwich-like domain characteristic of the ribokinase-like superfamily, and a second domain composed of four-stranded beta sheets. Structural comparison analysis suggested that residues G236, A237, G238, and D239 could be critical for ATP catalysis and substrate binding of PfkB. Our current work provides new insights into understanding the mechanism of the glycolysis in M. marinum. | |||
Structural analysis and functional study of phosphofructokinase B (PfkB) from Mycobacterium marinum.,Gao B, Ji R, Li Z, Su X, Li H, Sun Y, Ji C, Gan J, Li J Biochem Biophys Res Commun. 2021 Nov 19;579:129-135. doi:, 10.1016/j.bbrc.2021.09.051. Epub 2021 Sep 23. PMID:34597996<ref>PMID:34597996</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7fca" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Phosphofructokinase 3D structures|Phosphofructokinase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mycobacterium marinum M]] | ||
[[Category: Gao B]] | |||
[[Category: Ji R]] | |||
[[Category: Li J]] | |||