1omw: Difference between revisions

Revision as of 04:02, 3 May 2008

Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and gamma 2 subunits

OverviewOverview

The phosphorylation of heptahelical receptors by heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor kinases (GRKs) is a universal regulatory mechanism that leads to desensitization of G protein signaling and to the activation of alternative signaling pathways.We determined the crystallographic structure of bovine GRK2 in complex with G protein beta1gamma2 subunits.Our results show how the three domains of GRK2-the RGS (regulator of G protein signaling) homology, protein kinase, and pleckstrin homology domains-integrate their respective activities and recruit the enzyme to the cell membrane in an orientation that not only facilitates receptor phosphorylation, but also allows for the simultaneous inhibition of signaling by Galpha and Gbetagamma subunits.

About this StructureAbout this Structure

1OMW is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gbetagamma., Lodowski DT, Pitcher JA, Capel WD, Lefkowitz RJ, Tesmer JJ, Science. 2003 May 23;300(5623):1256-62. PMID:12764189 Page seeded by OCA on Sat May 3 04:02:38 2008

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OCA

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 [[Image:1omw.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/[Beta-adrenergic-receptor]_kinase [Beta-adrenergic-receptor] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.15 2.7.11.15] </span>
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-|GENE= GRK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), GNB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), GNG2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1omw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1omw OCA], [http://www.ebi.ac.uk/pdbsum/1omw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1omw RCSB]</span>
-}}
 '''Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and gamma 2 subunits'''
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 [[Category: Bos taurus]]
 [[Category: Protein complex]]
-[[Category: [Beta-adrenergic-receptor] kinase]]
 [[Category: Capel, W D.]]
 [[Category: Lefkowitz, R J.]]
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 [[Category: Pitcher, J A.]]
 [[Category: Tesmer, J J.G.]]
-[[Category: wd-40 repeat]]
+[[Category: Wd-40 repeat]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:02:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:47:32 2008''