7ef1: Difference between revisions
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==== | ==crystal structure of maize SHH2 SAWADEE domain in complex with and H3K9me1 peptide== | ||
<StructureSection load='7ef1' size='340' side='right'caption='[[7ef1]]' scene=''> | <StructureSection load='7ef1' size='340' side='right'caption='[[7ef1]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ef1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EF1 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ef1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ef1 OCA], [https://pdbe.org/7ef1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ef1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ef1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ef1 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ef1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ef1 OCA], [https://pdbe.org/7ef1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ef1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ef1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ef1 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B7ZYP9_MAIZE B7ZYP9_MAIZE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize. | |||
Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.,Wang Y, Zhou X, Luo J, Lv S, Liu R, Du X, Jia B, Yuan F, Zhang H, Du J J Integr Plant Biol. 2021 Jun;63(6):1091-1096. doi: 10.1111/jipb.13103. Epub 2021, Jun 3. PMID:33913587<ref>PMID:33913587</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ef1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Zea mays]] | ||
[[Category: Du J]] | |||
[[Category: Wang Y]] | |||