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==Crystal structure of Serine Racemase from Dictyostelium discoideum.== | ==Crystal structure of Serine Racemase from Dictyostelium discoideum.== | ||
<StructureSection load='6lut' size='340' side='right'caption='[[6lut]]' scene=''> | <StructureSection load='6lut' size='340' side='right'caption='[[6lut]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LUT OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6lut]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LUT FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lut OCA], [https://pdbe.org/6lut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lut RCSB], [https://www.ebi.ac.uk/pdbsum/6lut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lut ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/SRR_DICDI SRR_DICDI] May catalyze the synthesis of D-serine from L-serine. May have serine dehydratase activity (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Eukaryotic serine racemase (SR) is a pyridoxal 5'-phosphate enzyme belonging to the Fold-type II group, which catalyzes serine racemization and is responsible for the synthesis of D-Ser, a co-agonist of the N-methyl-d-aspartate receptor. In addition to racemization, SR catalyzes the dehydration of D- and L-Ser to pyruvate and ammonia. The bifuctionality of SR is thought to be important for D-Ser homeostasis. SR catalyzes the racemization of D- and L-Ser with almost the same efficiency. In contrast, the rate of L-Ser dehydration catalyzed by SR is much higher than that of D-Ser dehydration. This has caused the argument that SR does not catalyze the direct D-Ser dehydration and that D-Ser is first converted to L-Ser, then dehydrated. In this study, we investigated the substrate and solvent isotope effect of dehydration of D- and L-Ser catalyzed by SR from Dictyostelium discoideum (DdSR) and demonstrated that the enzyme catalyzes direct D-Ser dehydration. Kinetic studies of dehydration of four Thr isomers catalyzed by D. discoideum and mouse SRs suggest that SR discriminates the substrate configuration at C3 but not at C2. This is probably the reason for the difference in efficiency between L- and D-Ser dehydration catalyzed by SR. | |||
Mechanism of eukaryotic serine racemase-catalyzed serine dehydration.,Ito T, Matsuoka M, Goto M, Watanabe S, Mizobuchi T, Matsushita K, Nasu R, Hemmi H, Yoshimura T Biochim Biophys Acta Proteins Proteom. 2020 Sep;1868(9):140460. doi:, 10.1016/j.bbapap.2020.140460. Epub 2020 May 29. PMID:32474107<ref>PMID:32474107</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6lut" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Dictyostelium discoideum]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Goto M]] | [[Category: Goto M]] | ||
[[Category: Mizobuchi T]] | [[Category: Mizobuchi T]] | ||
[[Category: Yoshimura T]] | [[Category: Yoshimura T]] | ||