6luf: Difference between revisions

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<StructureSection load='6luf' size='340' side='right'caption='[[6luf]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
<StructureSection load='6luf' size='340' side='right'caption='[[6luf]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6luf]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LUF FirstGlance]. <br>
<table><tr><td colspan='2'>[[6luf]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O395 Vibrio cholerae O395]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LUF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6luf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6luf OCA], [http://pdbe.org/6luf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6luf RCSB], [http://www.ebi.ac.uk/pdbsum/6luf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6luf ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6luf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6luf OCA], [https://pdbe.org/6luf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6luf RCSB], [https://www.ebi.ac.uk/pdbsum/6luf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6luf ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A0H3AHP1_VIBC3 A0A0H3AHP1_VIBC3]
Bacterial enhancer-binding proteins (bEBPs) oligomerize through AAA(+) domains and use ATP hydrolysis-driven energy to isomerize the RNA polymerase-sigma(54) complex during transcriptional initiation. Here, we describe the first structure of the central AAA(+) domain of the flagellar regulatory protein FlrC (FlrC(C)), a bEBP that controls flagellar synthesis in Vibrio cholerae. Our results showed that FlrC(C) forms heptamer both in nucleotide (Nt)-free and -bound states without ATP-dependent subunit remodeling. Unlike the bEBPs such as NtrC1 or PspF, a novel cis-mediated "all or none" ATP binding occurs in the heptameric FlrC(C), because constriction at the ATPase site, caused by loop L3 and helix alpha7, restricts the proximity of the trans-protomer required for Nt binding. A unique "closed to open" movement of Walker A, assisted by trans-acting "Glu switch" Glu-286, facilitates ATP binding and hydrolysis. Fluorescence quenching and ATPase assays on FlrC(C) and mutants revealed that although Arg-349 of sensor II, positioned by trans-acting Glu-286 and Tyr-290, acts as a key residue to bind and hydrolyze ATP, Arg-319 of alpha7 anchors ribose and controls the rate of ATP hydrolysis by retarding the expulsion of ADP. Heptameric state of FlrC(C) is restored in solution even with the transition state mimicking ADP.AlF3. Structural results and pulldown assays indicated that L3 renders an in-built geometry to L1 and L2 causing sigma(54)-FlrC(C) interaction independent of Nt binding. Collectively, our results underscore a novel mechanism of ATP binding and sigma(54) interaction that strives to understand the transcriptional mechanism of the bEBPs, which probably interact directly with the RNA polymerase-sigma(54) complex without DNA looping.


Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC.,Dey S, Biswas M, Sen U, Dasgupta J J Biol Chem. 2015 Apr 3;290(14):8734-47. doi: 10.1074/jbc.M114.611434. Epub 2015 , Feb 16. PMID:25688103<ref>PMID:25688103</ref>
==See Also==
 
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6luf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chakraborty, S]]
[[Category: Vibrio cholerae O395]]
[[Category: Dasgupta, J]]
[[Category: Chakraborty S]]
[[Category: Flagellar synthesis]]
[[Category: Dasgupta J]]
[[Category: Mutation]]
[[Category: Transcription]]
[[Category: Vibrio cholerae]]

Latest revision as of 17:53, 29 November 2023

Trans-acting mutant Y290A of the central AAA+ domain of the flagellar regulatory protein FlrCTrans-acting mutant Y290A of the central AAA+ domain of the flagellar regulatory protein FlrC

Structural highlights

6luf is a 7 chain structure with sequence from Vibrio cholerae O395. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.45Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0H3AHP1_VIBC3

See Also

6luf, resolution 3.45Å

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