1ok8: Difference between revisions

Revision as of 03:57, 3 May 2008

CRYSTAL STRUCTURE OF THE DENGUE 2 VIRUS ENVELOPE GLYCOPROTEIN IN THE POSTFUSION CONFORMATION

OverviewOverview

Dengue virus enters a host cell when the viral envelope glycoprotein, E, binds to a receptor and responds by conformational rearrangement to the reduced pH of an endosome. The conformational change induces fusion of viral and host-cell membranes. A three-dimensional structure of the soluble E ectodomain (sE) in its trimeric, postfusion state reveals striking differences from the dimeric, prefusion form. The elongated trimer bears three 'fusion loops' at one end, to insert into the host-cell membrane. Their structure allows us to model directly how these fusion loops interact with a lipid bilayer. The protein folds back on itself, directing its carboxy terminus towards the fusion loops. We propose a fusion mechanism driven by essentially irreversible conformational changes in E and facilitated by fusion-loop insertion into the outer bilayer leaflet. Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry.

About this StructureAbout this Structure

1OK8 is a Single protein structure of sequence from Dengue virus 2. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the dengue virus envelope protein after membrane fusion., Modis Y, Ogata S, Clements D, Harrison SC, Nature. 2004 Jan 22;427(6972):313-9. PMID:14737159 Page seeded by OCA on Sat May 3 03:57:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ok8.gif|left|200px]]
- {{Structure
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-}}
 '''CRYSTAL STRUCTURE OF THE DENGUE 2 VIRUS ENVELOPE GLYCOPROTEIN IN THE POSTFUSION CONFORMATION'''
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 [[Category: Harrison, S C.]]
 [[Category: Modis, Y.]]
-[[Category: class 2 fusion protein,]]
+[[Category: Class 2 fusion protein]]
-[[Category: dengue virus]]
+[[Category: Dengue virus]]
-[[Category: flavivirus]]
+[[Category: Flavivirus]]
-[[Category: fusion peptide]]
+[[Category: Fusion peptide]]
-[[Category: low-ph conformational change]]
+[[Category: Low-ph conformational change]]
-[[Category: membrane fusion]]
+[[Category: Membrane fusion]]
-[[Category: trimer]]
+[[Category: Trimer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:57:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:46:36 2008''