1ohv: Difference between revisions
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[[Image:1ohv.gif|left|200px]] | [[Image:1ohv.gif|left|200px]] | ||
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'''4-AMINOBUTYRATE-AMINOTRANSFERASE FROM PIG''' | '''4-AMINOBUTYRATE-AMINOTRANSFERASE FROM PIG''' | ||
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==About this Structure== | ==About this Structure== | ||
1OHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry | 1OHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1gtx 1gtx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Storici, P.]] | [[Category: Storici, P.]] | ||
[[Category: 4-aminobutyric acid]] | [[Category: 4-aminobutyric acid]] | ||
[[Category: | [[Category: Aminotransferase]] | ||
[[Category: | [[Category: Antiepileptic drug target]] | ||
[[Category: | [[Category: Plp-dependent enzyme]] | ||
[[Category: | [[Category: Transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:52:04 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 03:52, 3 May 2008
4-AMINOBUTYRATE-AMINOTRANSFERASE FROM PIG
OverviewOverview
Gamma-aminobutyric acid aminotransferase (GABA-AT) is a pyridoxal 5'-phosphate-dependent enzyme responsible for the degradation of the inhibitory neurotransmitter GABA. GABA-AT is a validated target for antiepilepsy drugs because its selective inhibition raises GABA concentrations in brain. The antiepilepsy drug, gamma-vinyl-GABA (vigabatrin) has been investigated in the past by various biochemical methods and resulted in several proposals for its mechanisms of inactivation. In this study we solved and compared the crystal structures of pig liver GABA-AT in its native form (to 2.3-A resolution) and in complex with vigabatrin as well as with the close analogue gamma-ethynyl-GABA (to 2.3 and 2.8 A, respectively). Both inactivators form a covalent ternary adduct with the active site Lys-329 and the pyridoxal 5'-phosphate (PLP) cofactor. The crystal structures provide direct support for specific inactivation mechanisms proposed earlier on the basis of radio-labeling experiments. The reactivity of GABA-AT crystals with the two GABA analogues was also investigated by polarized absorption microspectrophotometry. The spectral data are discussed in relation to the proposed mechanism. Intriguingly, all three structures revealed a [2Fe-2S] cluster of yet unknown function at the center of the dimeric molecule in the vicinity of the PLP cofactors.
About this StructureAbout this Structure
1OHV is a Single protein structure of sequence from Sus scrofa. This structure supersedes the now removed PDB entry 1gtx. Full crystallographic information is available from OCA.
ReferenceReference
Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin., Storici P, De Biase D, Bossa F, Bruno S, Mozzarelli A, Peneff C, Silverman RB, Schirmer T, J Biol Chem. 2004 Jan 2;279(1):363-73. Epub 2003 Oct 8. PMID:14534310 Page seeded by OCA on Sat May 3 03:52:04 2008