1ogv: Difference between revisions

Revision as of 03:49, 3 May 2008

LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES

OverviewOverview

Well-ordered crystals of the bacterial photosynthetic reaction centre from Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we report the type I crystal packing that results from this crystallisation medium, for which 3D crystals grow as stacked 2D crystals, and the reaction centre X-ray structure is refined to 2.35A resolution. In this crystal form, the location of the membrane bilayer could be assigned with confidence. A cardiolipin-binding site is found at the protein-protein interface within the membrane-spanning region, shedding light on the formation of crystal contacts within the membrane. A chloride-binding site was identified in the membrane-spanning region, which suggests a putative site for interaction with the light-harvesting complex I, the cytochrome bc(1) complex or PufX. Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment.

About this StructureAbout this Structure

1OGV is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837 Page seeded by OCA on Sat May 3 03:49:45 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ogv.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ogv |SIZE=350|CAPTION= <scene name='initialview01'>1ogv</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_1ogv", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+M'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ogv|  PDB=1ogv  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ogv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ogv OCA], [http://www.ebi.ac.uk/pdbsum/1ogv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ogv RCSB]</span>
-}}
 '''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''
@@ Line 30: / Line 27: @@
 [[Category: Landau, E M.]]
 [[Category: Neutze, R.]]
-[[Category: bacteriochlorophyll]]
+[[Category: Bacteriochlorophyll]]
-[[Category: charge separation]]
+[[Category: Charge separation]]
-[[Category: electron transport]]
+[[Category: Electron transport]]
-[[Category: integral membrane protein]]
+[[Category: Integral membrane protein]]
-[[Category: lipid]]
+[[Category: Lipid]]
-[[Category: photosynthesis]]
+[[Category: Photosynthesis]]
-[[Category: reaction centre]]
+[[Category: Reaction centre]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:49:45 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:45:06 2008''