6k2h: Difference between revisions
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<StructureSection load='6k2h' size='340' side='right'caption='[[6k2h]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='6k2h' size='340' side='right'caption='[[6k2h]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6k2h]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6k2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2H FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2h OCA], [https://pdbe.org/6k2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2h RCSB], [https://www.ebi.ac.uk/pdbsum/6k2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q2FVM5_STAA8 Q2FVM5_STAA8] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Staphylococcus | [[Category: Staphylococcus aureus]] | ||
[[Category: Chen | [[Category: Chen W]] | ||
[[Category: Chen | [[Category: Chen X]] | ||
[[Category: Sangare | [[Category: Sangare L]] | ||
[[Category: Wang | [[Category: Wang C]] | ||
[[Category: Wu | [[Category: Wu M]] | ||
[[Category: Zang | [[Category: Zang J]] | ||
[[Category: Zhang | [[Category: Zhang X]] | ||
Latest revision as of 13:25, 22 November 2023
structural characterization of mutated NreA protein in nitrate binding site from staphylococcus aureus.structural characterization of mutated NreA protein in nitrate binding site from staphylococcus aureus.
Structural highlights
FunctionPublication Abstract from PubMedStaphylococcus aureus is an anaerobic facultative microorganism that features the NreABC system for nitrate respiration. NreB is the sensor histidine kinase that phosphorylates the response regulator NreC to stimulate the expression of target genes. NreA is a nitrate sensor which dissociates from NreB in the present of nitrate and relieves its inhibition on NreB. However, the molecular basis of how NreA regulate NreB remains unknown. In this study, we determined the crystal structures of nitrate-bound NreA from S. aureus (SaNreA/NO3(-)) and its apoNreA-like mutant SaNreAY(94A) in complex with ethanediol (SaNreA(Y94A)/EDO). Structural comparison reveals that the C-terminal loop in SaNreA/NO3(-) rearranges to an alpha-helix (alpha7) in SaNreA(Y94A)/EDO, which converts an acidic pocket on the surface to a positively charged region. This conformational change of SaNreA C-terminus might play a role in SaNreB binding. Structural insights into the conformational change of Staphylococcus aureus NreA at C-terminus.,Sangare L, Chen W, Wang C, Chen X, Wu M, Zhang X, Zang J Biotechnol Lett. 2020 Jan 22. pii: 10.1007/s10529-020-02807-2. doi:, 10.1007/s10529-020-02807-2. PMID:31970556[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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