1oey: Difference between revisions

Revision as of 03:45, 3 May 2008

HETERODIMER OF P40PHOX AND P67PHOX PB1 DOMAINS FROM HUMAN NADPH OXIDASE

OverviewOverview

Maximal activation of NADPH oxidase requires formation of a complex between the p40(phox) and p67(phox) subunits via association of their PB1 domains. We have determined the crystal structure of the p40(phox)/p67(phox) PB1 heterodimer, which reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40(phox) and basic residues in p67(phox). The structure enabled us to identify residues critical for heterodimerization among other members of the PB1 domain family, including the atypical protein kinase C zeta (PKC zeta) and its partners Par6 and p62 (ZIP, sequestosome). Both Par6 and p62 use their basic "back" to interact with the OPCA motif on the "front" of the PKC zeta. Besides heterodimeric interactions, some PB1 domains, like the p62 PB1, can make homotypic front-to-back arrays.

About this StructureAbout this Structure

1OEY is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62., Wilson MI, Gill DJ, Perisic O, Quinn MT, Williams RL, Mol Cell. 2003 Jul;12(1):39-50. PMID:12887891 Page seeded by OCA on Sat May 3 03:45:38 2008

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OCA

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 [[Image:1oey.jpg|left|200px]]
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 '''HETERODIMER OF P40PHOX AND P67PHOX PB1 DOMAINS FROM HUMAN NADPH OXIDASE'''
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 [[Category: Williams, R L.]]
 [[Category: Wilson, M I.]]
-[[Category: heterodimerization]]
+[[Category: Heterodimerization]]
-[[Category: nadph oxidase]]
+[[Category: Nadph oxidase]]
-[[Category: pb1 domain]]
+[[Category: Pb1 domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:45:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:44:22 2008''