5z4c: Difference between revisions
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==Crystal structure of Tailor== | ==Crystal structure of Tailor== | ||
<StructureSection load='5z4c' size='340' side='right' caption='[[5z4c]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='5z4c' size='340' side='right'caption='[[5z4c]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5z4c]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5z4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z4C FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z4c OCA], [https://pdbe.org/5z4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z4c RCSB], [https://www.ebi.ac.uk/pdbsum/5z4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z4c ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5z4c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5z4c" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[RNA uridylyltransferase|RNA uridylyltransferase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cheng | [[Category: Cheng L]] | ||
[[Category: Gong | [[Category: Gong Q]] | ||
[[Category: Jiang | [[Category: Jiang Y]] | ||
[[Category: Li | [[Category: Li F]] | ||
[[Category: Shi | [[Category: Shi Y]] | ||
[[Category: Xie | [[Category: Xie C]] | ||
[[Category: Yu | [[Category: Yu H]] | ||
Latest revision as of 11:45, 22 November 2023
Crystal structure of TailorCrystal structure of Tailor
Structural highlights
FunctionTUTT_DROME Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.[1] [2] Publication Abstract from PubMedTerminal uridylyl transferase (TUTase) is one type of enzyme that modifies RNA molecules by facilitating the post-transcriptional addition of uridyl ribonucleotides to their 3' ends. Recent researches have reported that Drosophila TUTase, Tailor, exhibits an intrinsic preference for RNA substrates ending in 3'G, distinguishing it from any other known TUTases. Through this unique feature, Tailor plays a crucial role as the repressor in the biogenesis pathway of splicing-derived mirtron pre-miRNAs. Here we describe crystal structures of core catalytic domain of Tailor and its complexes with RNA stretches 5'-AGU-3' and 5'-AGUU-3'. We demonstrate that R327 and N347 are two key residues contributing cooperatively to Tailor's preference for 3'G, and R327 may play an extra role in facilitating the extension of polyuridylation chain. We also demonstrate that conformational stability of the exit of RNA-binding groove also contributes significantly to Tailor's activity. Overall, our work reveals useful insights to explain why Drosophila Tailor can preferentially select RNA substrates ending in 3'G and provides important values for further understanding the biological significances of biogenesis pathway of mirtron in flies. Structural insights into a unique preference for 3' terminal guanine of mirtron in Drosophila TUTase tailor.,Cheng L, Li F, Jiang Y, Yu H, Xie C, Shi Y, Gong Q Nucleic Acids Res. 2018 Nov 8. pii: 5165274. doi: 10.1093/nar/gky1116. PMID:30407553[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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