1od9: Difference between revisions

Revision as of 03:41, 3 May 2008

N-TERMINAL OF SIALOADHESIN IN COMPLEX WITH ME-A-9-N-BENZOYL-AMINO-9-DEOXY-NEU5AC (BENZ COMPOUND)

OverviewOverview

The Siglec family of receptors mediates cell surface interactions through recognition of sialylated glycoconjugates. The crystal structure of the N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in complex with 2,3-sialyllactose has informed the design of sialic acid analogs (sialosides) that bind Siglecs with significantly enhanced affinities and specificities. Binding assays against sialoadhesin (Sn; Siglec-1), CD22 (Siglec-2), and MAG (Siglec-4) show a 10- to 300-fold reduction in IC(50) values (relative to methyl-alpha-Neu5Ac) for three sialosides bearing aromatic group modifications of the glycerol side chain: Me-alpha-9-N-benzoyl-amino-9-deoxy-Neu5Ac (BENZ), Me-alpha-9-N-(naphthyl-2-carbonyl)-amino-9-deoxy-Neu5Ac (NAP), and Me-alpha-9-N-(biphenyl-4-carbonyl)-amino-9-deoxy-Neu5Ac (BIP). Crystal structures of these sialosides in complex with SnD1 suggest explanations for the differences in specificity and affinity, providing further ideas for compound design of physiological and potentially therapeutic relevance.

About this StructureAbout this Structure

1OD9 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin., Zaccai NR, Maenaka K, Maenaka T, Crocker PR, Brossmer R, Kelm S, Jones EY, Structure. 2003 May;11(5):557-67. PMID:12737821 Page seeded by OCA on Sat May 3 03:41:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1od9.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1od9 |SIZE=350|CAPTION= <scene name='initialview01'>1od9</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1od9", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=BND:So4+Binding+Site+For+Chain+A'>BND</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BND:ME-A-N-BENZOYL-AMINO-9-DEOXY-NEU5AC'>BND</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1od9|  PDB=1od9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1od9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1od9 OCA], [http://www.ebi.ac.uk/pdbsum/1od9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1od9 RCSB]</span>
-}}
 '''N-TERMINAL OF SIALOADHESIN IN COMPLEX WITH ME-A-9-N-BENZOYL-AMINO-9-DEOXY-NEU5AC (BENZ COMPOUND)'''
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 [[Category: Maenaka, T.]]
 [[Category: Zaccai, N R.]]
-[[Category: carbohydrate binding]]
+[[Category: Carbohydrate binding]]
-[[Category: immune system]]
+[[Category: Immune system]]
-[[Category: immunoglobulin superfamily]]
+[[Category: Immunoglobulin superfamily]]
-[[Category: inhibitor design]]
+[[Category: Inhibitor design]]
-[[Category: siglec]]
+[[Category: Siglec]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:41:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:43:37 2008''