5xcv: Difference between revisions
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<StructureSection load='5xcv' size='340' side='right'caption='[[5xcv]], [[Resolution|resolution]] 2.14Å' scene=''> | <StructureSection load='5xcv' size='340' side='right'caption='[[5xcv]], [[Resolution|resolution]] 2.14Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5xcv]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XCV OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5xcv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus Rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XCV FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.143Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xcv OCA], [https://pdbe.org/5xcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xcv RCSB], [https://www.ebi.ac.uk/pdbsum/5xcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xcv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PDPN_HUMAN PDPN_HUMAN] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rattus]] | ||
[[Category: | [[Category: Arimori T]] | ||
[[Category: | [[Category: Takagi J]] | ||
Latest revision as of 10:59, 22 November 2023
Crystal structure of NZ-1 Fv-clasp fragment with its antigen peptideCrystal structure of NZ-1 Fv-clasp fragment with its antigen peptide
Structural highlights
FunctionPublication Abstract from PubMedAntibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this particular application. By fusing an anti-parallel coiled-coil structure derived from the SARAH domain of human Mst1 kinase to the variable region of an antibody, we succeeded in creating a novel chimeric antibody fragment of approximately 37 kDa, termed "Fv-clasp," which exhibits excellent crystallization compatibility while maintaining the binding ability of the original IgG molecule. The "clasp" and the engineered disulfide bond at the bottom of the Fv suppressed the internal mobility of the fragment and shielded hydrophobic residues, likely contributing to the high heat stability and the crystallizability of the Fv-clasp. Finally, Fv-clasp antibodies showed superior "chaperoning" activity over conventional Fab fragments, and facilitated the structure determination of an ectodomain fragment of integrin alpha6beta1. Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability.,Arimori T, Kitago Y, Umitsu M, Fujii Y, Asaki R, Tamura-Kawakami K, Takagi J Structure. 2017 Sep 6. pii: S0969-2126(17)30264-2. doi:, 10.1016/j.str.2017.08.011. PMID:28919443[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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