1oay: Difference between revisions

Revision as of 03:36, 3 May 2008

ANTIBODY MULTISPECIFICITY MEDIATED BY CONFORMATIONAL DIVERSITY

OverviewOverview

A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298 Page seeded by OCA on Sat May 3 03:36:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1oay.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1oay |SIZE=350|CAPTION= <scene name='initialview01'>1oay</scene>, resolution 2.66&Aring;
+The line below this paragraph, containing "STRUCTURE_1oay", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Fur+Binding+Site+For+Chain+J'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FUR:ACENAPHTHENEQUINONE'>FUR</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1oay|  PDB=1oay  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oay OCA], [http://www.ebi.ac.uk/pdbsum/1oay PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oay RCSB]</span>
-}}
 '''ANTIBODY MULTISPECIFICITY MEDIATED BY CONFORMATIONAL DIVERSITY'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-OAY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAY OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAY OCA].
 ==Reference==
 Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12610298 12610298]
-[[Category: Protein complex]]
-[[Category: Rattus rattus]]
 [[Category: James, L C.]]
 [[Category: Roversi, P.]]
 [[Category: Tawfik, D.]]
-[[Category: allergy]]
+[[Category: Allergy]]
-[[Category: antibody]]
+[[Category: Antibody]]
-[[Category: conformational diversity]]
+[[Category: Conformational diversity]]
-[[Category: ige]]
+[[Category: Ige]]
-[[Category: multispecificity]]
+[[Category: Multispecificity]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:36:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:42:33 2008''