1oar: Difference between revisions

Revision as of 03:36, 3 May 2008

FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED

OverviewOverview

A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.

About this StructureAbout this Structure

1OAR is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.

ReferenceReference

Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298 Page seeded by OCA on Sat May 3 03:36:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1oar.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1oar |SIZE=350|CAPTION= <scene name='initialview01'>1oar</scene>, resolution 2.23&Aring;
+The line below this paragraph, containing "STRUCTURE_1oar", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+N'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1oar|  PDB=1oar  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [http://www.ebi.ac.uk/pdbsum/1oar PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB]</span>
-}}
 '''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''
@@ Line 28: / Line 25: @@
 [[Category: Roversi, P.]]
 [[Category: Tawfik, D.]]
-[[Category: allergy]]
+[[Category: Allergy]]
-[[Category: antibody]]
+[[Category: Antibody]]
-[[Category: conformational diversity]]
+[[Category: Conformational diversity]]
-[[Category: ige]]
+[[Category: Ige]]
-[[Category: multispecificity]]
+[[Category: Multispecificity]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:36:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:42:37 2008''