7pnt: Difference between revisions
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==== | ==Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Tfb1m== | ||
<StructureSection load='7pnt' size='340' side='right'caption='[[7pnt]]' scene=''> | <StructureSection load='7pnt' size='340' side='right'caption='[[7pnt]], [[Resolution|resolution]] 3.19Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7pnt]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PNT FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pnt OCA], [https://pdbe.org/7pnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pnt RCSB], [https://www.ebi.ac.uk/pdbsum/7pnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pnt ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.19Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5F0:(3~{S})-3-azanyl-4-methoxy-4-oxidanylidene-butanoic+acid'>5F0</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pnt OCA], [https://pdbe.org/7pnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pnt RCSB], [https://www.ebi.ac.uk/pdbsum/7pnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pnt ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/RT02_MOUSE RT02_MOUSE] Required for mitoribosome formation and stability, and mitochondrial translation.[UniProtKB:Q9Y399] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. (1)) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex(2) that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome. | |||
Mechanism of mitoribosomal small subunit biogenesis and preinitiation.,Itoh Y, Khawaja A, Laptev I, Cipullo M, Atanassov I, Sergiev P, Rorbach J, Amunts A Nature. 2022 Jun;606(7914):603-608. doi: 10.1038/s41586-022-04795-x. Epub 2022 , Jun 8. PMID:35676484<ref>PMID:35676484</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7pnt" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: Amunts A]] | |||
[[Category: Itoh Y]] | |||
[[Category: Khawaja A]] | |||
[[Category: Laptev I]] | |||
[[Category: Rorbach J]] | |||
[[Category: Sergiev P]] | |||
Latest revision as of 14:05, 15 November 2023
Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Tfb1mAssembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Tfb1m
Structural highlights
FunctionRT02_MOUSE Required for mitoribosome formation and stability, and mitochondrial translation.[UniProtKB:Q9Y399] Publication Abstract from PubMedMitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. (1)) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex(2) that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome. Mechanism of mitoribosomal small subunit biogenesis and preinitiation.,Itoh Y, Khawaja A, Laptev I, Cipullo M, Atanassov I, Sergiev P, Rorbach J, Amunts A Nature. 2022 Jun;606(7914):603-608. doi: 10.1038/s41586-022-04795-x. Epub 2022 , Jun 8. PMID:35676484[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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