1o29: Difference between revisions

Revision as of 03:16, 3 May 2008

Crystal structure of Thymidylate Synthase Complementing Protein (TM0449) from Thermotoga maritima with FAD and FdUMP at 2.0 A resolution

OverviewOverview

Like thymidylate synthase (TS) in eukaryotes, the thymidylate synthase-complementing proteins (TSCPs) are mandatory for cell survival of many prokaryotes in the absence of external sources of thymidylate. Details of the mechanism of this novel family of enzymes are unknown. Here, we report the structural and functional analysis of a TSCP from Thermotoga maritima and its complexes with substrate, analogs, and cofactor. The structures presented here provide a basis for rationalizing the TSCP catalysis and reveal the possibility of the design of an inhibitor. We have identified a new helix-loop-strand FAD binding motif characteristic of the enzymes in the TSCP family. The presence of a hydrophobic core with residues conserved among the TSCP family suggests a common overall fold.

About this StructureAbout this Structure

1O29 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein., Mathews II, Deacon AM, Canaves JM, McMullan D, Lesley SA, Agarwalla S, Kuhn P, Structure. 2003 Jun;11(6):677-90. PMID:12791256 Page seeded by OCA on Sat May 3 03:16:01 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1o29.jpg|left|200px]]
- {{Structure
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-|GENE= TM0449 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK00847 thyX]</span>
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-|RELATEDENTRY=[[1o24|1O24]], [[1o25|1O25]], [[1o26|1O26]], [[1o27|1O27]], [[1o28|1O28]], [[1o2a|1O2A]], [[1o2b|1O2B]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o29 OCA], [http://www.ebi.ac.uk/pdbsum/1o29 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1o29 RCSB]</span>
-}}
 '''Crystal structure of Thymidylate Synthase Complementing Protein (TM0449) from Thermotoga maritima with FAD and FdUMP at 2.0 A resolution'''
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 [[Category: Mathews, I I.]]
 [[Category: McMullan, D.]]
-[[Category: jcsg]]
+[[Category: Jcsg]]
-[[Category: joint center for structural genomic]]
+[[Category: Joint center for structural genomic]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: thymidylate synthase complementing protein]]
+[[Category: Thymidylate synthase complementing protein]]
-[[Category: tm0449]]
+[[Category: Tm0449]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:16:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:38:45 2008''