5mkc: Difference between revisions
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<StructureSection load='5mkc' size='340' side='right'caption='[[5mkc]], [[Resolution|resolution]] 2.04Å' scene=''> | <StructureSection load='5mkc' size='340' side='right'caption='[[5mkc]], [[Resolution|resolution]] 2.04Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5mkc]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5mkc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MKC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mkc OCA], [https://pdbe.org/5mkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mkc RCSB], [https://www.ebi.ac.uk/pdbsum/5mkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mkc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0H2UNT6_STRPN A0A0H2UNT6_STRPN] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bonnet | [[Category: Streptococcus pneumoniae]] | ||
[[Category: Cartannaz | [[Category: Bonnet J]] | ||
[[Category: Contreras-Martel | [[Category: Cartannaz J]] | ||
[[Category: | [[Category: Contreras-Martel C]] | ||
[[Category: | [[Category: Di Guilmi AM]] | ||
[[Category: Kleman | [[Category: Fenel D]] | ||
[[Category: Morlot | [[Category: Kleman JP]] | ||
[[Category: Schoehn | [[Category: Morlot C]] | ||
[[Category: Tourcier | [[Category: Schoehn G]] | ||
[[Category: Vernet | [[Category: Tourcier G]] | ||
[[Category: Vernet T]] | |||
Latest revision as of 21:47, 1 November 2023
Crystal structure of the RrgA Jo.In complexCrystal structure of the RrgA Jo.In complex
Structural highlights
FunctionPublication Abstract from PubMedUnusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 A. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding. Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.,Bonnet J, Cartannaz J, Tourcier G, Contreras-Martel C, Kleman JP, Morlot C, Vernet T, Di Guilmi AM Sci Rep. 2017 Mar 2;7:43564. doi: 10.1038/srep43564. PMID:28252635[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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