1nyb: Difference between revisions

Revision as of 03:07, 3 May 2008

SOLUTION STRUCTURE OF THE BACTERIOPHAGE PHI21 N PEPTIDE-BOXB RNA COMPLEX

OverviewOverview

We determined the solution structure of a 22-amino-acid peptide from the amino-terminal domain of the bacteriophage phi21 N protein in complex with its cognate 24-mer boxB RNA hairpin using heteronuclear magnetic resonance spectroscopy. The N peptide binds as an alpha-helix and interacts predominately with the major groove side of the 5' half of the boxB RNA stem-loop. This binding interface is defined by surface complementarity of polar and nonpolar interactions, and little sequence-specific recognition. The phi21 boxB loop (CUAACC) has hydrogen bond and backbone torsions typical of the "U-turn" motif, as well as base stacking of the last 4 nt, and a hydrogen bonded C:C pair closing the loop. The exposed face of the phi21 boxB loop, in complex with the N peptide, is strikingly similar to the GNRA tetraloop-like folds of the related lambda and P22 bacteriophage N peptide-boxB RNA complexes. The N peptide-boxB complexes of the various phage, while individually distinct, provide similar structural features for interactions with the Escherichia coli host factors to enable antitermination.

About this StructureAbout this Structure

1NYB is a Single protein structure of sequence from Enterobacteria phage phi21. Full crystallographic information is available from OCA.

ReferenceReference

Structural mimicry in the phage phi21 N peptide-boxB RNA complex., Cilley CD, Williamson JR, RNA. 2003 Jun;9(6):663-76. PMID:12756325 Page seeded by OCA on Sat May 3 03:07:50 2008

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OCA

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 [[Image:1nyb.gif|left|200px]]
- {{Structure
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-|GENE= N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10737 Enterobacteria phage phi21])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyb OCA], [http://www.ebi.ac.uk/pdbsum/1nyb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nyb RCSB]</span>
-}}
 '''SOLUTION STRUCTURE OF THE BACTERIOPHAGE PHI21 N PEPTIDE-BOXB RNA COMPLEX'''
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 [[Category: Cilley, C D.]]
 [[Category: Williamson, J R.]]
-[[Category: peptide-rna complex]]
+[[Category: Peptide-rna complex]]
-[[Category: transcription antitermination]]
+[[Category: Transcription antitermination]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:07:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:37:16 2008''